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Dynamic complex optical fields for optical manipulation, 3D microscopy and photostimulation of neurotransmitters

Daria, Vincent; Stricker, Christian; Bekkers, John; Redman, Stephen; Bachor, Hans

Description

We demonstrate a multi-functional system capable of multiple-site two-photon excitation of photo-sensitive compounds as well as transfer of optical mechanical properties on an array of mesoscopic particles. We use holographic projection of a single Ti:Sapphire laser operating in femtosecond pulse mode to show that the projected three-dimensional light patterns have sufficient spatiotemporal photon density for multi-site two-photon excitation of biological fluorescent markers and caged...[Show more]

dc.contributor.authorDaria, Vincent
dc.contributor.authorStricker, Christian
dc.contributor.authorBekkers, John
dc.contributor.authorRedman, Stephen
dc.contributor.authorBachor, Hans
dc.coverage.spatialSan Diego, CA
dc.date.accessioned2015-12-13T22:59:13Z
dc.date.available2015-12-13T22:59:13Z
dc.date.createdAugust 1-5 2010
dc.identifier.isbn9780819482587
dc.identifier.urihttp://hdl.handle.net/1885/83670
dc.description.abstractWe demonstrate a multi-functional system capable of multiple-site two-photon excitation of photo-sensitive compounds as well as transfer of optical mechanical properties on an array of mesoscopic particles. We use holographic projection of a single Ti:Sapphire laser operating in femtosecond pulse mode to show that the projected three-dimensional light patterns have sufficient spatiotemporal photon density for multi-site two-photon excitation of biological fluorescent markers and caged neurotransmitters. Using the same laser operating in continuous-wave mode we can use the same light patterns for non-invasive transfer of both linear and orbital angular momentum on a variety of mesoscopic particles. The system also incorporates high-speed scanning using acousto-optic modulators to rapidly render 3D images of neuron samples via two-photon microscopy.
dc.publisherSPIE - The International Society for Optical Engineering
dc.relation.ispartofseriesOptical Trapping and Optical Micromanipulation VII
dc.sourceProceedings of SPIE - The International Society for Optical Engineering
dc.subjectKeywords: 3-D image; 3-D microscopy; Acousto-optic modulator; Continuous wave modes; Dynamic microscopy; Femtosecond pulse; Fluorescent markers; High speed scanning; Holographic projection; Light patterns; Mesoscopic particles; Multi-photon absorption; Multi-site; Dynamic microscopy; Holographic projection; Multi-photon absorption; Multiple-beam optical tweezers
dc.titleDynamic complex optical fields for optical manipulation, 3D microscopy and photostimulation of neurotransmitters
dc.typeConference paper
local.description.notesImported from ARIES
local.description.refereedYes
dc.date.issued2010
local.identifier.absfor069900 - OTHER BIOLOGICAL SCIENCES
local.identifier.absfor110900 - NEUROSCIENCES
local.identifier.ariespublicationf5625xPUB11952
local.type.statusMetadata only
local.contributor.affiliationDaria, Vincent, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationStricker, Christian, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationBekkers, John, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationRedman, Stephen, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationBachor, Hans, College of Physical and Mathematical Sciences, ANU
local.identifier.doi10.1117/12.862388
local.identifier.absseo970102 - Expanding Knowledge in the Physical Sciences
dc.date.updated2016-02-24T08:39:58Z
local.identifier.scopusID2-s2.0-77958113692
CollectionsANU Research Publications

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