The role of cysteine residues in the sulphate transporter, SHST1:Construction of a functional cysteine-less transporter
We investigated the role of cysteine residues in the sulphate transporter, SHST1, with the aim of generating a functional cysteine-less variant. SHST1 contains five cysteine residues and none was essential for function. However, replacement of C421 resulted in a reduction in transport activity. Sulphate transport by C205 mutants was dependent on the size of the residue at this position. Alanine at position 205 resulted in a complete loss of function whereas leucine resulted in a 3-fold increase...[Show more]
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|Source:||Biochimica et Biophysica Acta: International journal of Biochemistry and Biophysics|
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