Skip navigation
Skip navigation

Acute skin irritant effects of cyanobacteria (blue-green algae) in healthy volunteers

Pilotto, Louis; Hobson, Peter; Burch, Michael; Ranmuthugala, Geethanjali; Attewell, Robyn; Weightman, Warren

Description

Objective: To assess the skin irritant potential of a range of laboratory grown cyanobacterial species using skin-patch testing on human volunteers. Methods: Cell suspensions and extracts of cyanobacterial cultures of Microcystis aeruginosa (non-toxic strain), Anabaena circinalis and Nodularia spumigena were applied to 64 volunteers in one trial, and Microcystis aeruginosa (toxic strain), Apanocapsa incerta and Cylindrospermopsis raciborskii were applied to 50 volunteers in a second trial. Six...[Show more]

dc.contributor.authorPilotto, Louis
dc.contributor.authorHobson, Peter
dc.contributor.authorBurch, Michael
dc.contributor.authorRanmuthugala, Geethanjali
dc.contributor.authorAttewell, Robyn
dc.contributor.authorWeightman, Warren
dc.date.accessioned2015-12-13T22:56:25Z
dc.identifier.issn1326-0200
dc.identifier.urihttp://hdl.handle.net/1885/82788
dc.description.abstractObjective: To assess the skin irritant potential of a range of laboratory grown cyanobacterial species using skin-patch testing on human volunteers. Methods: Cell suspensions and extracts of cyanobacterial cultures of Microcystis aeruginosa (non-toxic strain), Anabaena circinalis and Nodularia spumigena were applied to 64 volunteers in one trial, and Microcystis aeruginosa (toxic strain), Apanocapsa incerta and Cylindrospermopsis raciborskii were applied to 50 volunteers in a second trial. Six cell concentrations of each organism in the range from less than 5,000 to greater than 200,000 cells/mL were applied in random order using adhesive skin patches (Finn Chambers®). In addition, the applications included two treatments of each cyanobacterial species, involving whole and lysed cells, and positive (sodium lauryl sulphate) and negative (culture media) controls. Patches were removed after 24 hours and assessment of erythema was made by a dermatologist blinded to the species, cell type and concentration. Results: On average, between 20% and 24% of individuals with 95% confidence interval ±8% reacted across the concentration range tested for these cyanobacterial species. The reaction rates were lower (11% to 15%) among the subset of subjects not reacting to negative controls. The reaction was mostly mild, and in all cases was resolved without treatment. This was the case for both whole and lysed cells with little difference in reaction rates between these two treatments. There was also no dose-response across the concentration range for any of the cyanobacterial species tested. Conclusion: A small proportion of healthy people (around 20%) may develop a skin reaction to cyanobacteria in the course of normal water recreation, but the reaction is mild and resolved without treatment.
dc.publisherPublic Health Association of Australia
dc.sourceAustralian and New Zealand Journal of Public Health
dc.subjectKeywords: dodecyl sulfate sodium; Anabaena; article; bacterial count; bacterial growth; bacterium culture; blue green alga; cell suspension; cell type; concentration response; controlled study; culture medium; Cyanobacterium; cytolysis; dermatology; disease severit
dc.titleAcute skin irritant effects of cyanobacteria (blue-green algae) in healthy volunteers
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume28
dc.date.issued2004
local.identifier.absfor111706 - Epidemiology
local.identifier.ariespublicationMigratedxPub10994
local.type.statusPublished Version
local.contributor.affiliationPilotto, Louis, University of New South Wales
local.contributor.affiliationHobson, Peter, Australian Water Quality Centre
local.contributor.affiliationBurch, Michael, Australian Water Quality Centre
local.contributor.affiliationRanmuthugala, Geethanjali, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationAttewell, Robyn, Covance Pty Ltd
local.contributor.affiliationWeightman, Warren, Australian Water Quality Centre
local.description.embargo2037-12-31
local.bibliographicCitation.issue3
local.bibliographicCitation.startpage220
local.bibliographicCitation.lastpage224
dc.date.updated2015-12-11T11:15:27Z
local.identifier.scopusID2-s2.0-3042809868
CollectionsANU Research Publications

Download

File Description SizeFormat Image
01_Pilotto_Acute_skin_irritant_effects_of_2004.pdf36.56 kBAdobe PDF    Request a copy


Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  20 July 2017/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator