The influence of structure and composition of a reverse SDS microemulsion on enzymatic activities and electrical conductivities
-
Altmetric Citations
Bauduin, Pierre; Touraud, Didier; Kunz, Werner; Savelli, Marie-Pierre; Pulvin, Sylviane; Ninham, Barry
Description
The activity of the enzyme horse radish peroxidase (HRP) is studied in a series of reverse microemulsions composed of dodecane, aqueous buffer, sodium dodecylsufate (SDS) and alcohols of the homologous series 1-butanol to 1-octanol. The HRP catalyzed reaction is the oxidation of a classical water soluble substrate, the 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) by hydrogen peroxide. In parallel electrical conductivity measurements are performed on the same...[Show more]
dc.contributor.author | Bauduin, Pierre | |
---|---|---|
dc.contributor.author | Touraud, Didier | |
dc.contributor.author | Kunz, Werner | |
dc.contributor.author | Savelli, Marie-Pierre | |
dc.contributor.author | Pulvin, Sylviane | |
dc.contributor.author | Ninham, Barry | |
dc.date.accessioned | 2015-12-13T22:56:21Z | |
dc.identifier.issn | 0021-9797 | |
dc.identifier.uri | http://hdl.handle.net/1885/82770 | |
dc.description.abstract | The activity of the enzyme horse radish peroxidase (HRP) is studied in a series of reverse microemulsions composed of dodecane, aqueous buffer, sodium dodecylsufate (SDS) and alcohols of the homologous series 1-butanol to 1-octanol. The HRP catalyzed reaction is the oxidation of a classical water soluble substrate, the 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) by hydrogen peroxide. In parallel electrical conductivity measurements are performed on the same solutions. The structural changes in the microemulsions, as inferred by the conductivity measurements, correlate remarkably well with the changes in the enzymatic activities. In particular it is found that (a) the maximum activity of the enzyme is always related to its optimum hydration and that this hydration can be related to the microemulsion structures, (b) the enzyme inhibition caused by the alcohols in microemulsions is a consequence of both the solubility of the alcohols in the buffer and the rigidity of the interfacial film. Consequently, it can be concluded that enzymatic activity measurements are a valuable tool to study confined systems such as microemulsions and, in particular, the amount of available hydration water. Enzymatic activities can be finely tuned by small changes in microemulsion structures, probably in a predictive way. | |
dc.publisher | Academic Press | |
dc.source | Journal of Colloid and Interface Science | |
dc.subject | Keywords: 1-octanol; Hydration water; Microemulsion structures; Alcohols; Enzymes; Hydration; Oxidation; Microemulsions; 2,2 azinobis(3 ethylbenzothiazoline 6 sulfonic acid)diammonium; alcohol; anionic surfactant; buffer; butanol; dodecane; dodecyl sulfate sodium; Alcohols; Anionic surfactant; Conductivity; Enzymatic reaction; Horse radish peroxidase; Hydration; Reverse microemulsion | |
dc.title | The influence of structure and composition of a reverse SDS microemulsion on enzymatic activities and electrical conductivities | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.description.refereed | Yes | |
local.identifier.citationvolume | 292 | |
dc.date.issued | 2005 | |
local.identifier.absfor | 030606 - Structural Chemistry and Spectroscopy | |
local.identifier.ariespublication | MigratedxPub10977 | |
local.type.status | Published Version | |
local.contributor.affiliation | Bauduin, Pierre, University of Regensburg | |
local.contributor.affiliation | Touraud, Didier, University of Regensburg | |
local.contributor.affiliation | Kunz, Werner, University of Regensburg | |
local.contributor.affiliation | Savelli, Marie-Pierre, Faculte de Pharmacie d'Amiens | |
local.contributor.affiliation | Pulvin, Sylviane, Universite de Technologie de Compiegne | |
local.contributor.affiliation | Ninham, Barry, College of Physical and Mathematical Sciences, ANU | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.startpage | 244 | |
local.bibliographicCitation.lastpage | 254 | |
local.identifier.doi | 10.1016/j.jcis.2005.05.043 | |
dc.date.updated | 2015-12-11T11:15:13Z | |
local.identifier.scopusID | 2-s2.0-27544479093 | |
Collections | ANU Research Publications |
Download
File | Description | Size | Format | Image |
---|---|---|---|---|
01_Bauduin_The_influence_of_structure_and_2005.pdf | 536.49 kB | Adobe PDF | Request a copy |
Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.
Updated: 17 November 2022/ Responsible Officer: University Librarian/ Page Contact: Library Systems & Web Coordinator