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Molecular cloning and characterization of the Strictosidine synthase Gene from Rauwolfia verticillata

Chen, Rong; Liao, Zhihua; Chen, Min; Wang, Q.; Yang, C.X.; Yang, Y.J.

Description

The strictosidine synthase (STR, EC 4.3.3.2) catalyzes the condensation of tryptamine and secologanin to form strictosidine, which is the universal precursor for a wide range of pharmaceutical terpenoid indole alkaloids (TIAs). The full-length cDNA encoding STR was cloned and characterized from Rauwolfia verticillata a Chinese native plant producing TIAs, such as reserpine and ajmalicine. The new cDNA was designed as RvSTR and submitted to GenBank to get an accession number DQ017054. The...[Show more]

dc.contributor.authorChen, Rong
dc.contributor.authorLiao, Zhihua
dc.contributor.authorChen, Min
dc.contributor.authorWang, Q.
dc.contributor.authorYang, C.X.
dc.contributor.authorYang, Y.J.
dc.date.accessioned2015-12-13T22:55:14Z
dc.identifier.issn1021-4437
dc.identifier.urihttp://hdl.handle.net/1885/82444
dc.description.abstractThe strictosidine synthase (STR, EC 4.3.3.2) catalyzes the condensation of tryptamine and secologanin to form strictosidine, which is the universal precursor for a wide range of pharmaceutical terpenoid indole alkaloids (TIAs). The full-length cDNA encoding STR was cloned and characterized from Rauwolfia verticillata a Chinese native plant producing TIAs, such as reserpine and ajmalicine. The new cDNA was designed as RvSTR and submitted to GenBank to get an accession number DQ017054. The full-length cDNA of RvSTR was 1211 bp containing a 1035-bp open reading frame encoding a deduced 344-amino-acid polypeptide with a calculated mol wt of 38.2 kD and an isoelectric point of 5.19. Comparative and bioinformatic analysis revealed that RvSTR showed a higher similarity to STRs from Apocynaceae species, including Catharanthus roseus and Ophiorrhiza pumila, but a relatively lower similarity to other plant STRs. The unique essential catalytic residue Glu-309 was conserved in all alignment plant species. The phylogenetic analysis revealed that STRs were divided into two groups, including plant and bacterial enzymes. The tissue expression pattern analysis indicated that RvSTR expression could be detected in all tested organs of R. verticillata, including roots, stems, leaves, fruits, and flowers. The lowest transcription level was observed in flowers and the highest was found in fruits; subsequently, the order of transcription level decrease was stems > roots > leaves. The cloning and characterization of RvSTR give a new STR sequence involved in TIA biosynthesis of R. verticillata, and provide a candidate gene for metabolic engineering of the TIA pathway in R. verticillata.
dc.publisherMaik Nauka/Interperiodica Publishing
dc.sourceRussian Journal of Plant Physiology
dc.subjectKeywords: Apocynaceae; Bacteria (microorganisms); Catharanthus roseus; Ophiorrhiza pumila; Rauvolfia; Verticillata Cloning; Expression profile; Rauwolfia verticillata; STR gene
dc.titleMolecular cloning and characterization of the Strictosidine synthase Gene from Rauwolfia verticillata
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume55
dc.date.issued2008
local.identifier.absfor060112 - Structural Biology (incl. Macromolecular Modelling)
local.identifier.ariespublicationf5625xPUB10696
local.type.statusPublished Version
local.contributor.affiliationChen, Rong, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationLiao, Zhihua, Sothwest University
local.contributor.affiliationChen, Min, Southwest University
local.contributor.affiliationWang, Q., China Rural Technology Development Center
local.contributor.affiliationYang, C.X., Southwest University
local.contributor.affiliationYang, Y.J., Southwest University
local.description.embargo2037-12-31
local.bibliographicCitation.issue5
local.bibliographicCitation.startpage670
local.bibliographicCitation.lastpage675
local.identifier.doi10.1134/S1021443708050117
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
dc.date.updated2016-02-24T08:36:45Z
local.identifier.scopusID2-s2.0-56749163578
local.identifier.thomsonID000258845600011
CollectionsANU Research Publications

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