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Weak alignment of paramagnetic proteins warrants correction for residual CSA effects in measurements of pseudocontact shifts

John, Michael; Park, Ah Young; Pintacuda, Guido; Dixon, Nicholas; Otting, Gottfried


Paramagnetic metal ions can induce molecular alignment with respect to the magnetic field. This alignment generates residual anisotropic chemical shifts (RACS) due to nonisotropic averaging over the molecular orientations. Using a 30 kDa protein-protein complex, the RACS effects are shown to be significant for heteronuclear spins with large chemical shift anisotropies, lanthanide ions with large anisotropic magnetic susceptibility tensors, and measurements at high magnetic field. Therefore,...[Show more]

CollectionsANU Research Publications
Date published: 2005
Type: Journal article
Source: Journal of the American Chemical Society
DOI: 10.1021/ja0564259


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