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Proteomic Analysis of Somatic Embryogenesis in Medicago truncatula. ExPlant Cultures Grown under 6-Benzylaminopurine and 1-Naphthaleneacetic Acid Treatments

Imin, Nijat; Nizamidin, Mahira; Daniher, Daniel; Nolan, Kim E; Rose, Ray J; Rolfe, Barry

Description

The Medicago truncatula line 2HA has a 500-fold greater capacity to regenerate plants in culture by somatic embryogenesis than wild-type Jemalong. We have compared proteomes of tissue cultures from leaf explants of these two lines. Both 2HA and Jemalong explants were grown on media containing the auxin 1-naphthaleneacetic acid and the cytokinin 6-benzylaminopurine. Proteins were extracted from the cultures at different time points (2, 5, and 8 weeks), separated by two-dimensional gel...[Show more]

CollectionsANU Research Publications
Date published: 2005
Type: Journal article
URI: http://hdl.handle.net/1885/82193
Source: Plant Physiology
DOI: 10.1104/pp.104.055277

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