Temporal and spatial expression of hexose transporters in developing tomato (Lycopersicon esculentum) fruit
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Dibley, Stephen J; Gear, Michael L; Yang, Xiao; Rosche, Elke; Offler, Christina E; McCurdy, David W; Patrick, John W
Description
Correlative physiological evidence suggests that membrane transport into storage parenchyma cells is a key step in determining hexose levels accumulated in tomato (Lycopersicon esculentum Mill.) fruit (Ruan et al. 1997). Expression of three previously identified hexose transporter genes (LeHT1, 2 and 3) demonstrated that LeHT3, and to a lesser extent LeHT1, are the predominant transporters expressed in young fruit (10 d after anthesis; DAA). Expression of both transporters dropped sharply until...[Show more]
dc.contributor.author | Dibley, Stephen J | |
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dc.contributor.author | Gear, Michael L | |
dc.contributor.author | Yang, Xiao | |
dc.contributor.author | Rosche, Elke | |
dc.contributor.author | Offler, Christina E | |
dc.contributor.author | McCurdy, David W | |
dc.contributor.author | Patrick, John W | |
dc.date.accessioned | 2015-12-13T22:54:37Z | |
dc.date.available | 2015-12-13T22:54:37Z | |
dc.identifier.issn | 1445-4408 | |
dc.identifier.uri | http://hdl.handle.net/1885/82183 | |
dc.description.abstract | Correlative physiological evidence suggests that membrane transport into storage parenchyma cells is a key step in determining hexose levels accumulated in tomato (Lycopersicon esculentum Mill.) fruit (Ruan et al. 1997). Expression of three previously identified hexose transporter genes (LeHT1, 2 and 3) demonstrated that LeHT3, and to a lesser extent LeHT1, are the predominant transporters expressed in young fruit (10 d after anthesis; DAA). Expression of both transporters dropped sharply until 24 DAA, after which only LeHT3 expression remained at detectable levels through to fruit ripening. LeHT2 was not expressed substantially until the onset of fruit ripening. For fruit at both 10 and 30 DAA, LeHT3 transcripts were detected in storage parenchyma cells of the outer pericarp tissue, but not in vascular bundles or the first layer of parenchyma cells surrounding these bundles. In contrast to LeHT gene expression, hexose transporter protein levels were maximal between 20 and 30 DAA, which corresponded to the period of highest hexose accumulation. The delayed appearance of transporter protein is consistent with some form of post-transcriptional regulation. Based on these analyses, LeHT3 appears to be responsible for the rapid hexose accumulation in developing tomato fruit. | |
dc.publisher | CSIRO Publishing | |
dc.source | Functional Plant Biology | |
dc.subject | Keywords: Cells; Genes; Tissue; Gene expression; Hexose transporters; Menbrane transport; Sugar accumulation; Fruits; metabolism; Cells; Fruits; Tissue; Tomatoes; Lycopersicon esculentum Fruit; Gene expression; Hexose transporters; Membrane transport; Sugar accumulation; Tomato | |
dc.title | Temporal and spatial expression of hexose transporters in developing tomato (Lycopersicon esculentum) fruit | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.description.refereed | Yes | |
local.identifier.citationvolume | 32 | |
dc.date.issued | 2005 | |
local.identifier.absfor | 060405 - Gene Expression (incl. Microarray and other genome-wide approaches) | |
local.identifier.absfor | 070601 - Horticultural Crop Growth and Development | |
local.identifier.ariespublication | MigratedxPub10461 | |
local.type.status | Published Version | |
local.contributor.affiliation | Dibley, Stephen J, University of Newcastle | |
local.contributor.affiliation | Gear, Michael L, University of Newcastle | |
local.contributor.affiliation | Yang, Xiao, University of Newcastle | |
local.contributor.affiliation | Rosche, Elke, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Offler, Christina E, University of Newcastle | |
local.contributor.affiliation | McCurdy, David W, University of Newcastle | |
local.contributor.affiliation | Patrick, John W, University of Newcastle | |
local.bibliographicCitation.startpage | 777 | |
local.bibliographicCitation.lastpage | 785 | |
local.identifier.doi | 10.1071/FP04224 | |
dc.date.updated | 2015-12-11T11:05:20Z | |
local.identifier.scopusID | 2-s2.0-26444510271 | |
Collections | ANU Research Publications |
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