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Interaction of the replication terminator protein of Bacillus subtilis with DNA probed by NMR spectroscopy

Hastings, Adam; Folmer, Rutger; Duggin, Iain; Wake, R. Gerry; Wilce, Matthew; Wilce, Jacqueline; Otting, Gottfried

Description

Termination of DNA replication in Bacillus subtilis involves the polar arrest of replication forks by a specific complex formed between the dimeric 29 kDa replication terminator protein (RTP) and DNA terminator sites. We have used NMR spectroscopy to probe the changes in1H-15N correlation spectra of a15N-labelled RTP.C110S mutant upon the addition of a 21 base pair symmetrical DNA binding site. Assignment of the1H-15N correlations was achieved using a suite of triple resonance NMR experiments...[Show more]

CollectionsANU Research Publications
Date published: 2005
Type: Journal article
URI: http://hdl.handle.net/1885/81995
Source: Biochemical and Biophysical Research Communications
DOI: 10.1016/j.bbrc.2005.07.082

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