Following directed evolution with crystallography: structural changes observed in changing the substrate specificity of dienelactone hydrolase
The enzyme dienelactone hydrolase (DLH) has undergone directed evolution to produce a series of mutant proteins that have enhanced activity towards the non-physiological substrates α-naphthyl acetate and p-nitrophenyl acetate. In terms of steady-state ki
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|Source:||Acta Crystallographica Section D: Biological Crystallography|
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