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Reduced low-voltage activated K+ conductances and enhanced central excitability in a congenitally deaf (dn/dn) mouse

Leao, Richardson; Berntson Eichner, Amy; Forsythe, Ian D; Walmsley, Bruce

Description

We have investigated changes in the neuronal excitability of the auditory brainstem in a congenitally deaf mouse (deafness dn/dn). Whole cell patch recordings from principal neurones of the medial nucleus of the trapezoid body (MNTB) showed strikingly enhanced excitability in the deaf mice when compared to control CBA mice at 12-14 days postnatal. MNTB neurones in normal CBA mice showed the phenotypic single action potential response on depolarization in current clamp; however, recordings from...[Show more]

dc.contributor.authorLeao, Richardson
dc.contributor.authorBerntson Eichner, Amy
dc.contributor.authorForsythe, Ian D
dc.contributor.authorWalmsley, Bruce
dc.date.accessioned2015-12-13T22:50:58Z
dc.date.available2015-12-13T22:50:58Z
dc.identifier.issn0022-3751
dc.identifier.urihttp://hdl.handle.net/1885/81052
dc.description.abstractWe have investigated changes in the neuronal excitability of the auditory brainstem in a congenitally deaf mouse (deafness dn/dn). Whole cell patch recordings from principal neurones of the medial nucleus of the trapezoid body (MNTB) showed strikingly enhanced excitability in the deaf mice when compared to control CBA mice at 12-14 days postnatal. MNTB neurones in normal CBA mice showed the phenotypic single action potential response on depolarization in current clamp; however, recordings from CBA mice carrying the homozygous deafness mutation fired trains of action potentials on depolarization. We show here that these changes are associated with reduced functional expression of dendrotoxin-sensitive Kv1 potassium channels. In contrast, no differences were found in voltage-gated calcium currents between control and deaf mice. These results reveal that loss of hair cell function in the cochlea leads to changes in ion channel expression in the central nervous system and suggests that this deafness model will be an important tool in understanding central changes occurring in human congenital deafness and in exploring activity-dependent regulation of ion channel expression.
dc.publisherCambridge University Press
dc.sourceJournal of Physiology
dc.subjectKeywords: calcium; dendrotoxin; ion channel; potassium ion; voltage gated calcium channel; action potential; animal cell; animal experiment; animal model; article; auditory system; brain nerve cell; brain stem; cell function; central nervous system; cochlea; congen
dc.titleReduced low-voltage activated K+ conductances and enhanced central excitability in a congenitally deaf (dn/dn) mouse
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume559
dc.date.issued2004
local.identifier.absfor110902 - Cellular Nervous System
local.identifier.ariespublicationMigratedxPub9381
local.type.statusPublished Version
local.contributor.affiliationLeao, Richardson, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationBerntson Eichner, Amy, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationForsythe, Ian D, University of Leicester
local.contributor.affiliationWalmsley, Bruce, College of Medicine, Biology and Environment, ANU
local.bibliographicCitation.issue1
local.bibliographicCitation.startpage25
local.bibliographicCitation.lastpage33
local.identifier.doi10.1113/jphysiol.2004.067421
dc.date.updated2015-12-11T10:43:09Z
local.identifier.scopusID2-s2.0-4444324052
CollectionsANU Research Publications

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