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probing for pH-regulated proteins in Sinorhizobium medicae using proteomic analysis

Reeve, W; Tiwari, Ravi; Guerreiro, N; Stubbs, J; Dilworth, M; Glenn, Andrew R; Rolfe, Barry; Djordjevic, Michael; Howieson, John G


To elucidate the mechanisms of pH response in an acid-tolerant Sinorhizobium medicae strain we have identifi ed acid-activated gene transcription and now complement this approach by using a proteomic analysis to identify the changes that occur following exposure to acidity. Protein profi les of persistently or transiently acid-stressed S. medicae cells were compared to those grown in pH neutral, buffered media. Fifty pH-regulated proteins were identified; N-terminal sequences for 15 of these...[Show more]

CollectionsANU Research Publications
Date published: 2004
Type: Journal article
Source: Journal of Molecular Microbiology and Biotechnology
DOI: 10.1159/000078657


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