Conversion of the Escherichia coli Cytochrome b562 to an Archetype Cytochrome b: A Mutant with Bis-Histidine Ligation of Heme Iron
A mutant of the Escherichia coli cytochrome b562 has been created in which the heme-ligating methionine (Met) at position 7 has been replaced with a histidine (His) (M7H). This protein is a double mutant that also has the His 63 to asparagine (H63N) mutation, which removes a solvent-exposed His. While the H63N mutation has no measurable effect on the cytochrome, the M7H mutation converts the atypical His/Met heme ligation in cytochrome b 562 to the classic cytochrome b-type bis-His ligation....[Show more]
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