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Hofmeister specific-ion effects on enzyme activity and buffer pH: Horseradish peroxidase in citrate buffer

Bauduin, Pierre; Nohmie, Fawaz; Touraud, Didier; Neueder, Roland; Kunz, Werner; Ninham, Barry

Description

Salt addition to enzymes in buffer always induce the problem of the respective influences of electrostatic interactions and anion specificity on buffer pH and enzyme kinetics. In the present paper the influence of some sodium salts (Na2SO4, NaCl, NaBr and NaNO3) on the pH of a citrate buffer (c = 0.025 M), and on the catalytic constants of horseradish peroxidase (HRP) is studied. First, the pH changes due to the presence of salts in the buffer are examined; second, catalytic constants, KmABTS,...[Show more]

CollectionsANU Research Publications
Date published: 2006
Type: Journal article
URI: http://hdl.handle.net/1885/80514
Source: Journal of Molecular Liquids
DOI: 10.1016/j.molliq.2005.03.003

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