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COBRA, an Arabidopsis Extracellular Glycosyl-Phosphatidyl Inositol-Anchored Protein, Specifically Controls Highly Anisotropic Expansion through its Involvement in Cellulose Microfibril Orientation

Roudier, Francois; Fernandez, Anita G; Fujita, Machiko; Himmelspach, Regina; Borner, Georg H H; Schindelman, Gary; Song, Shuang; Baskin, Tobias I; Dupree, Paul; Wasteneys, Geoffrey; Benfey, Philip N

Description

The orientation of cell expansion is a process at the heart of plant morphogenesis. Cellulose microfibrils are the primary anisotropic material in the cell wall and thus are likely to be the main determinant of the orientation of cell expansion. COBRA (COB) has been identified previously as a potential regulator of cellulose biogenesis. In this study, characterization of a null allele, cob-4, establishes the key role of COB in controlling anisotropic expansion in most developing organs....[Show more]

dc.contributor.authorRoudier, Francois
dc.contributor.authorFernandez, Anita G
dc.contributor.authorFujita, Machiko
dc.contributor.authorHimmelspach, Regina
dc.contributor.authorBorner, Georg H H
dc.contributor.authorSchindelman, Gary
dc.contributor.authorSong, Shuang
dc.contributor.authorBaskin, Tobias I
dc.contributor.authorDupree, Paul
dc.contributor.authorWasteneys, Geoffrey
dc.contributor.authorBenfey, Philip N
dc.date.accessioned2015-12-13T22:48:54Z
dc.identifier.issn1040-4651
dc.identifier.urihttp://hdl.handle.net/1885/80280
dc.description.abstractThe orientation of cell expansion is a process at the heart of plant morphogenesis. Cellulose microfibrils are the primary anisotropic material in the cell wall and thus are likely to be the main determinant of the orientation of cell expansion. COBRA (COB) has been identified previously as a potential regulator of cellulose biogenesis. In this study, characterization of a null allele, cob-4, establishes the key role of COB in controlling anisotropic expansion in most developing organs. Quantitative polarized-light and field-emission scanning electron microscopy reveal that loss of anisotropic expansion in cob mutants is accompanied by disorganization of the orientation of cellulose microfibrils and subsequent reduction of crystalline cellulose. Analyses of the conditional cob-1 allele suggested that COB is primarily implicated in microfibril deposition during rapid elongation. Immunodetection analysis in elongating root cells revealed that, in agreement with its substitution by a glycosylphosphatidylinositol anchor, COB was polarly targeted to both the plasma membrane and the longitudinal cell walls and was distributed in a banding pattern perpendicular to the longitudinal axis via a microtubule-dependent mechanism. Our observations suggest that COB, through its involvement in cellulose microfibril orientation, is an essential factor in highly anisotropic expansion during plant morphogenesis.
dc.publisherAmerican Society of Plant Biologists
dc.sourceThe Plant Cell
dc.subjectKeywords: Biological membranes; Cells; Cellulose; Crystalline materials; Anisotropic expansion; Cellulose microfibrils; Immunodetection; Rapid elongation; Proteins; Cells; Cellulose; Crystallites; Membranes; Proteins; Arabidopsis; Arabidopsis protein; cellulose; CO
dc.titleCOBRA, an Arabidopsis Extracellular Glycosyl-Phosphatidyl Inositol-Anchored Protein, Specifically Controls Highly Anisotropic Expansion through its Involvement in Cellulose Microfibril Orientation
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume17
dc.date.issued2005
local.identifier.absfor070601 - Horticultural Crop Growth and Development
local.identifier.ariespublicationMigratedxPub8558
local.type.statusPublished Version
local.contributor.affiliationRoudier, Francois, Duke University
local.contributor.affiliationFernandez, Anita G, New York University
local.contributor.affiliationFujita, Machiko, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHimmelspach, Regina, University of British Columbia
local.contributor.affiliationBorner, Georg H H, University of Cambridge
local.contributor.affiliationSchindelman, Gary, New York University
local.contributor.affiliationSong, Shuang, Duke University
local.contributor.affiliationBaskin, Tobias I, University of Massachusetts
local.contributor.affiliationDupree, Paul, University of Cambridge
local.contributor.affiliationWasteneys, Geoffrey, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationBenfey, Philip N, Duke University
local.description.embargo2037-12-31
local.bibliographicCitation.startpage1749
local.bibliographicCitation.lastpage1763
local.identifier.doi10.1105/tpc.105.031732
dc.date.updated2015-12-11T10:31:25Z
local.identifier.scopusID2-s2.0-28444480365
CollectionsANU Research Publications

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