Wilson, Ryan; Baillie, Brett; Jones, David
The discovery of an endoplasmic reticulum (ER) retrieval motif at the C-terminus of the predicted Cf-9 resistance protein suggested that Cf-9 might function in the ER. To test whether Cf-9 could detect its cognate avirulence protein, Avr9, from the ER, variants of Avr9 with the ER retrieval motifs KDEL or HDEL were transiently expressed in Cf-9 tobacco. Cf-9-mediated necrosis in response to Avr9 tagged at the C-terminus with the KDEL motif was substantially delayed compared with unmodified...[Show more]
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