Raised level of cyclin dependent kinase A after prolonged suspension culture of Nicotiana plumbaginifolia is associated with more rapid growth and division, diminished cytoskeleton and lost capacity for regeneration: implications for instability of cultured plant cells
A long maintained suspension culture of Nicotiana plumbaginifolia, compared with a newer culture, had increased levels of cyclin dependent kinase A (CDKA;1) a halved cell doubling time and twice the stationary cell density. The newer culture downregulated CDKA protein levels by 75% during the transition to stationary phase and, in mixtures of natural and synthetic auxins with cytokinins, developed shoot tissue that could regenerate into plants, while the older cell line developed only callus....[Show more]
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|Source:||The Plant Cell|
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