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Cell-free synthesis of 15 N-labeled proteins for NMR studies

Ozawa, Kiyoshi; Dixon, Nicholas; Otting, Gottfried


Modern cell-free in vitro protein synthesis systems present powerful tools for the synthesis of isotope-labeled proteins in high yields. The production of selectively 15N-labeled proteins from 15N-labeled amino acids is particularly economic and yields are often sufficient to analyze the proteins very quickly by two-dimensional NMR spectra recorded of the crude reaction mixture without concentration or chromatographic purification of the protein. We review methodological aspects of cell-free in...[Show more]

CollectionsANU Research Publications
Date published: 2005
Type: Journal article
Source: IUBMB Life
DOI: 10.1080/15216540500217859


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