A Novel Dynamin-Related Protein Has Been Recruited for Apicoplast Fission in Toxoplasma gondii
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van Dooren, Giel; Reiff, Sarah B.; Tomova, Cveta; Meissner, Markus; Humbel, Bruno M.; Striepen, Boris
Description
Background: Apicomplexan parasites cause numerous important human diseases, including malaria and toxoplasmosis. Apicomplexa belong to the Alveolata, a group that also includes ciliates and dinoflagellates. Apicomplexa retain a plastid organelle (the apicoplast) that was derived from an endosymbiotic relationship between the alveolate ancestor and a red alga. Apicoplasts are essential for parasite growth and must correctly divide and segregate into daughter cells upon cytokinesis. Apicoplast...[Show more]
dc.contributor.author | van Dooren, Giel | |
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dc.contributor.author | Reiff, Sarah B. | |
dc.contributor.author | Tomova, Cveta | |
dc.contributor.author | Meissner, Markus | |
dc.contributor.author | Humbel, Bruno M. | |
dc.contributor.author | Striepen, Boris | |
dc.date.accessioned | 2015-12-13T22:45:26Z | |
dc.identifier.issn | 0960-9822 | |
dc.identifier.uri | http://hdl.handle.net/1885/79782 | |
dc.description.abstract | Background: Apicomplexan parasites cause numerous important human diseases, including malaria and toxoplasmosis. Apicomplexa belong to the Alveolata, a group that also includes ciliates and dinoflagellates. Apicomplexa retain a plastid organelle (the apicoplast) that was derived from an endosymbiotic relationship between the alveolate ancestor and a red alga. Apicoplasts are essential for parasite growth and must correctly divide and segregate into daughter cells upon cytokinesis. Apicoplast division depends on association with the mitotic spindle, although little is known about the molecular machinery involved in this process. Apicoplasts lack the conserved machinery that divides chloroplasts in plants and red algae, suggesting that these mechanisms are unique. Results: Here, we demonstrate that a dynamin-related protein in Toxoplasma gondii (TgDrpA) localizes to punctate regions on the apicoplast surface. We generate a conditional dominant-negative TgDrpA cell line to disrupt TgDrpA functions and demonstrate that TgDrpA is essential for parasite growth and apicoplast biogenesis. Fluorescence recovery after photobleaching and time-lapse imaging studies provide evidence for a direct role for TgDrpA in apicoplast fission. Conclusions: Our data suggest that DrpA was likely recruited from the alveolate ancestor to function in fission of the symbiont and ultimately replaced the conserved division machinery of that symbiont. | |
dc.publisher | Cell Press | |
dc.source | Current Biology | |
dc.subject | Keywords: dynamin; hybrid protein; microtubule associated protein; protozoal protein; animal; article; cell culture; cell organelle; classification; cytology; fibroblast; genetics; human; metabolism; phylogeny; Toxoplasma; ultrastructure; Animals; Cells, Cultured; CELLBIO; EVO_ECOL | |
dc.title | A Novel Dynamin-Related Protein Has Been Recruited for Apicoplast Fission in Toxoplasma gondii | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 19 | |
dc.date.issued | 2009 | |
local.identifier.absfor | 060104 - Cell Metabolism | |
local.identifier.ariespublication | f5625xPUB8159 | |
local.type.status | Published Version | |
local.contributor.affiliation | van Dooren, Giel, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Reiff, Sarah B., University of Georgia | |
local.contributor.affiliation | Tomova, Cveta, Utrecht University | |
local.contributor.affiliation | Meissner, Markus, Heidelberg University School of Medicine | |
local.contributor.affiliation | Humbel, Bruno M., Utrecht University | |
local.contributor.affiliation | Striepen, Boris, University of Georgia | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.issue | 4 | |
local.bibliographicCitation.startpage | 267 | |
local.bibliographicCitation.lastpage | 276 | |
local.identifier.doi | 10.1016/j.cub.2008.12.048 | |
local.identifier.absseo | 920109 - Infectious Diseases | |
dc.date.updated | 2016-02-24T09:39:59Z | |
local.identifier.scopusID | 2-s2.0-60349117040 | |
local.identifier.thomsonID | 000263779600018 | |
Collections | ANU Research Publications |
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