Skip navigation
Skip navigation

Cell-free protein synthesis in an autoinduction system for NMR studies of protein-protein interactions

Ozawa, Kiyoshi; Jergic, Slobodan; Crowther, Jeffrey; Thompson, Phillip; Wijffels, Gene; Otting, Gottfried; Dixon, Nicholas

Description

Cell-free protein synthesis systems provide facile access to proteins in a nascent state that enables formation of soluble, native protein-protein complexes even if one of the protein components is prone to self-aggregation and precipitation. Combined with selective isotope-labeling, this allows the rapid analysis of protein-protein interactions with few15N-HSQC spectra. The concept is demonstrated with binary and ternary complexes between the χ, ψ and γ subunits of Escherichia coli DNA...[Show more]

CollectionsANU Research Publications
Date published: 2005
Type: Journal article
URI: http://hdl.handle.net/1885/79730
Source: Journal of Biomolecular NMR
DOI: 10.1007/s10858-005-7946-4

Download

File Description SizeFormat Image
01_Ozawa_Cell-free_protein_synthesis_in_2005.pdf310.96 kBAdobe PDF    Request a copy


Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  20 July 2017/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator