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Cell-free protein synthesis in an autoinduction system for NMR studies of protein-protein interactions

Ozawa, Kiyoshi; Jergic, Slobodan; Crowther, Jeffrey; Thompson, Phillip; Wijffels, Gene; Dixon, Nicholas; Otting, Gottfried


Cell-free protein synthesis systems provide facile access to proteins in a nascent state that enables formation of soluble, native protein-protein complexes even if one of the protein components is prone to self-aggregation and precipitation. Combined with selective isotope-labeling, this allows the rapid analysis of protein-protein interactions with few15N-HSQC spectra. The concept is demonstrated with binary and ternary complexes between the χ, ψ and γ subunits of Escherichia coli DNA...[Show more]

CollectionsANU Research Publications
Date published: 2005
Type: Journal article
Source: Journal of Biomolecular NMR
DOI: 10.1007/s10858-005-7946-4


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