Cell-free protein synthesis in an autoinduction system for NMR studies of protein-protein interactions
Cell-free protein synthesis systems provide facile access to proteins in a nascent state that enables formation of soluble, native protein-protein complexes even if one of the protein components is prone to self-aggregation and precipitation. Combined with selective isotope-labeling, this allows the rapid analysis of protein-protein interactions with few15N-HSQC spectra. The concept is demonstrated with binary and ternary complexes between the χ, ψ and γ subunits of Escherichia coli DNA...[Show more]
|Collections||ANU Research Publications|
|Source:||Journal of Biomolecular NMR|
|01_Ozawa_Cell-free_protein_synthesis_in_2005.pdf||310.96 kB||Adobe PDF||Request a copy|
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