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Beta-catenin/TCF4 transactivates miR-30e during intestinal cell differentiation

Liao, Yalin; Lonnerdal, Bo

Description

The Wnt/beta-catenin/TCF4 pathway plays critical roles in the maintenance of small intestinal epithelium; however, downstream targets of the beta-catenin/TCF4 complex are not extensively characterized. We identified miR-30e as an immediate target activated by the beta-catenin/TCF4 complex. miR-30e was detected in the peri-nuclear region of the intestinal crypt IEC-6 cells. Bioinformatics analysis revealed clustered beta-catenin/TCF4 binding sites within the miR-30e promoter region. This...[Show more]

dc.contributor.authorLiao, Yalin
dc.contributor.authorLonnerdal, Bo
dc.date.accessioned2015-12-13T22:44:01Z
dc.identifier.issn1420-682X
dc.identifier.urihttp://hdl.handle.net/1885/79462
dc.description.abstractThe Wnt/beta-catenin/TCF4 pathway plays critical roles in the maintenance of small intestinal epithelium; however, downstream targets of the beta-catenin/TCF4 complex are not extensively characterized. We identified miR-30e as an immediate target activated by the beta-catenin/TCF4 complex. miR-30e was detected in the peri-nuclear region of the intestinal crypt IEC-6 cells. Bioinformatics analysis revealed clustered beta-catenin/TCF4 binding sites within the miR-30e promoter region. This promoter region was cloned into pGL3-control luciferase reporter vector, with the enhancer region removed. Transfection of pCMV-SPORT6-beta-catenin expression vector dose-dependently increased luciferase activity, and co-transfection of pCMV-SPORT6-TCF4 expression vector further enhanced the promoter activity. Dexamethasone-induced IEC-6 cells differentiation caused a 2.5-fold increase in miR-30e expression, and upon beta-catenin siRNA transfection, miR-30e increased 1.3-fold. Electrophoretic mobility shift assay and chromatin immunoprecipitation assay confirmed the binding between beta-catenin/TCF4 complexes from IEC-6 nuclear extracts and the putative sequences in the miR-30e promoter. These results demonstrate that beta-catenin/TCF4 transactivates miR-30e during intestinal cell differentiation.
dc.publisherBirkhauser Verlag
dc.sourceCellular and Molecular Life Sciences
dc.subjectKeywords: beta catenin; luciferase; microRNA; microRNA 30e; T cell factor 4 protein; T cell factor protein; unclassified drug; animal cell; article; binding affinity; binding site; cell differentiation; chromatin immunoprecipitation; complex formation; controlled s Beta-catenin; Differentiation; IEC-6 cells; Intestine; MiR-30e; TCF4
dc.titleBeta-catenin/TCF4 transactivates miR-30e during intestinal cell differentiation
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume67
dc.date.issued2010
local.identifier.absfor060100 - BIOCHEMISTRY AND CELL BIOLOGY
local.identifier.ariespublicationf5625xPUB7899
local.type.statusPublished Version
local.contributor.affiliationLiao, Yalin, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationLonnerdal, Bo, University of California
local.description.embargo2037-12-31
local.bibliographicCitation.issue17
local.bibliographicCitation.startpage2969
local.bibliographicCitation.lastpage2978
local.identifier.doi10.1007/s00018-010-0366-y
dc.date.updated2016-02-24T09:37:59Z
local.identifier.scopusID2-s2.0-77956729743
CollectionsANU Research Publications

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