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On-column refolding of recombinant human interleukin-4 from inclusion bodies

Razeghifard, Mohammad


Interleukin-4 (IL4) is a multifunctional cytokine which plays a key role in the immune system. Several antagonists/agonists of IL4 are reported through mutagenesis studies, but their solution structural studies using nuclear magnetic resonance (NMR) spectroscopy are hindered as milligram quantities of isotopically labeled protein are required for structural refinements. In this work, a His-tagged recombinant form of human IL4 was overexpressed in Escherichia coli under the control of a T7...[Show more]

CollectionsANU Research Publications
Date published: 2004
Type: Journal article
Source: Protein Expression and Purification
DOI: 10.1016/j.pep.2004.05.007


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