Skip navigation
Skip navigation

Advances in cell-free protein synthesis for NMR sample preparation

Kralicek, Andrew V; Ozawa, Kiyoshi

Description

Cell-based expression strategies exist for the2H,13C and15N stable-isotope enrichment of target proteins for NMR studies. However, there are no guarantees that the protein will express to a reasonable level or be folded correctly. In addition, the economic cost of producing an isotope-labeled protein may be prohibitive because of the scale of production required or the cost of isotope-labeling sources. Many of these problems may be alleviated by the use of cell-free protein synthesis systems....[Show more]

CollectionsANU Research Publications
Date published: 2011
Type: Book chapter
URI: http://hdl.handle.net/1885/79022
Book Title: Biomolecular NMR Spectroscopy
DOI: 10.3233/978-1-60750-695-9-3

Download

File Description SizeFormat Image
01_Kralicek_Advances_in_cell-free_protein_2011.pdf517.56 kBAdobe PDF    Request a copy


Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  19 May 2020/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator