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Adaptation of a species-specific multiplex PCR assay for the identification of blood meal source in Culicoides (Ceratopogonidae: Diptera): Applications on Palaearctic biting midge species, vectors of Orbiviruses

Garros, Claire; Gardes, Laetitia; Allene, Xavier; Rakotoarivony, Ignace; Viennet, Elvina; Rossi, Sophie; Balenghien, T

Description

Culicoides are small biting midges involved worldwide in the transmission of bluetongue and African horse sickness viruses. Feeding behaviours of Palaearctic biting midge species and their spatio-temporal dynamics remain unclear at the specific level. Three multiplex species-specific PCR-based assays were developed and used to identify blood meal source of engorged females of Palaearctic midge species of veterinary interest. Species-specific primers of potential hosts from livestock, domestic...[Show more]

CollectionsANU Research Publications
Date published: 2011
Type: Journal article
URI: http://hdl.handle.net/1885/78656
Source: Infection, Genetics and Evolution
DOI: 10.1016/j.meegid.2011.04.002

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