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Identification of novel three allergens from Anisakis simplex by chemiluminescent immunoscreening of an expression cDNA library

Kobayashi, Yukihiro; Ohsaki, Kenichi; Ikeda, Kaori; Kakemoto, Seiko; Ishizaki, Shoichiro; Shimakura, Kuniyoshi; Nagashima, Yuji; Shiomi, Kazuo


Anisakis simplex is a representative nematode parasitizing marine organisms, such as fish and squids, and causes not only anisakiasis but also IgE-mediated allergy. Although 10 kinds of proteins have so far been identified as A. simplex allergens, many unknown allergens are considered to still exist. In this study, a chemiluminescent immunoscreening method with higher sensitivity than the conventional method was developed and used to isolate IgE-positive clones from an expression cDNA library...[Show more]

CollectionsANU Research Publications
Date published: 2011
Type: Journal article
Source: Parasitology International
DOI: 10.1016/j.parint.2011.01.004


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