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Proteomic characterization of human milk whey proteins during a twelve-month lactation period

Liao, Yalin; Alvarado, Rudy; Phinney, Brett; Lonnerdal, Bo

Description

Human milk is a rich source of bioactive proteins that support the early growth and development of the newborn. Although the major components of the protein fraction in human milk have been studied, the expression and relative abundance of minor components have received limited attention. We examined the expression of low-abundance proteins in the whey fraction of human milk and their dynamic changes over a twelve-month lactation period. The low-abundance proteins were enriched by ProteoMiner...[Show more]

dc.contributor.authorLiao, Yalin
dc.contributor.authorAlvarado, Rudy
dc.contributor.authorPhinney, Brett
dc.contributor.authorLonnerdal, Bo
dc.date.accessioned2015-12-13T22:41:35Z
dc.identifier.issn1535-3893
dc.identifier.urihttp://hdl.handle.net/1885/78574
dc.description.abstractHuman milk is a rich source of bioactive proteins that support the early growth and development of the newborn. Although the major components of the protein fraction in human milk have been studied, the expression and relative abundance of minor components have received limited attention. We examined the expression of low-abundance proteins in the whey fraction of human milk and their dynamic changes over a twelve-month lactation period. The low-abundance proteins were enriched by ProteoMiner beads, and protein identification was performed by liquid chromatography tandem mass spectrometry. One hundred and fifteen proteins were identified, thirty-eight of which have not been previously reported in human colostrum or milk. We also for the first time described differences in protein patterns among the low-abundance proteins during lactation. These results enhance our knowledge about the complexity of the human milk proteome, which constitutes part of the advantages to the breast-fed infant.
dc.publisherAmerican Chemical Society
dc.sourceJournal of Proteome Research
dc.subjectKeywords: milk protein; proteome; amino acid sequence; article; breast milk; colostrum; lactation; molecular dynamics; priority journal; protein analysis; protein expression; proteomics; unindexed sequence; whey; Chromatography, Liquid; Databases, Protein; Female; human milk; LC-MS/MS; protein; proteomics; whey
dc.titleProteomic characterization of human milk whey proteins during a twelve-month lactation period
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume10
dc.date.issued2011
local.identifier.absfor110500 - DENTISTRY
local.identifier.ariespublicationf5625xPUB7193
local.type.statusPublished Version
local.contributor.affiliationLiao, Yalin, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationAlvarado, Rudy, University of California
local.contributor.affiliationPhinney, Brett, University of California
local.contributor.affiliationLonnerdal, Bo, University of California
local.description.embargo2037-12-31
local.bibliographicCitation.issue4
local.bibliographicCitation.startpage1746
local.bibliographicCitation.lastpage1754
local.identifier.doi10.1021/pr101028k
dc.date.updated2016-02-24T09:33:28Z
local.identifier.scopusID2-s2.0-79953697144
CollectionsANU Research Publications

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