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Site-specific Labelling with a Metal Chelator for Protein-structure Refinement

Pintacuda, Guido; Moshref, Ahmad; Leonchiks, Ainars; Sharipo, Anatoly; Otting, Gottfried

Description

A single free Cys sidechain in the N-terminal domain of the E. coli arginine repressor was covalently derivatized with S-cysteaminyl-EDTA for site-specific attachment of paramagnetic metal ions. The effects of chelated metal ions were monitored with 15N-HSQC spectra. Complexation of Co2+, which has a fast relaxing electron spin, resulted in significant pseudocontact shifts, but also in peak doubling which was attributed to the possibility of forming two different stereoisomers of the EDTA-Co2+...[Show more]

CollectionsANU Research Publications
Date published: 2004
Type: Journal article
URI: http://hdl.handle.net/1885/78459
Source: Journal of Biomolecular NMR
DOI: 10.1023/B:JNMR.0000032610.17058.fe

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