Overexpression and characterization of a novel (S)-specific extended short-chain dehydrogenase/reductase from Candida parapsilosis
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Wang, Qiuyan; Shen, Linghong; Ye, Tingting; Cao, Dan; Chen, Rong; Pei, Xiaolin; Xie, Tian; Li, Yan; Gong, Weibo; Yen, Xiaopu
Description
The gene encoding a putative protein from Candida parapsilosis CDC317 (CPE) was cloned and overexpressed in Escherichia coli. The protein was amenable to overexpression in E. coli and constituted 35% of the total cell protein content. The optimal activity was determined at pH 5.5 and 40°C with the substrate 4-chloro-3-oxobutanoate ethyl ester (COBE). The optical purity of the product was over 99% enantiomeric excess for the (S)-isomer, and the molar conversion yield of the product was 91.1%....[Show more]
dc.contributor.author | Wang, Qiuyan | |
---|---|---|
dc.contributor.author | Shen, Linghong | |
dc.contributor.author | Ye, Tingting | |
dc.contributor.author | Cao, Dan | |
dc.contributor.author | Chen, Rong | |
dc.contributor.author | Pei, Xiaolin | |
dc.contributor.author | Xie, Tian | |
dc.contributor.author | Li, Yan | |
dc.contributor.author | Gong, Weibo | |
dc.contributor.author | Yen, Xiaopu | |
dc.date.accessioned | 2015-12-13T22:40:57Z | |
dc.identifier.issn | 0960-8524 | |
dc.identifier.uri | http://hdl.handle.net/1885/78283 | |
dc.description.abstract | The gene encoding a putative protein from Candida parapsilosis CDC317 (CPE) was cloned and overexpressed in Escherichia coli. The protein was amenable to overexpression in E. coli and constituted 35% of the total cell protein content. The optimal activity was determined at pH 5.5 and 40°C with the substrate 4-chloro-3-oxobutanoate ethyl ester (COBE). The optical purity of the product was over 99% enantiomeric excess for the (S)-isomer, and the molar conversion yield of the product was 91.1%. The apparent km value for COBE was 0.19±0.01mM, which is an order of magnitude lower than that of other enzymes in the literature. | |
dc.publisher | Elsevier | |
dc.source | Bioresource Technology | |
dc.subject | Keywords: Apparent k; Asymmetric reduction; Candida parapsilosis; Cell protein content; Chiral alcohols; E. coli; Enantiomeric excess; Ethyl esters; Extended short-chain dehydrogenase reductase; Molar conversion; Optical purity; Optimal activity; Over-expression; P Asymmetric reduction; Chiral alcohol; Extended short-chain dehydrogenase reductase | |
dc.title | Overexpression and characterization of a novel (S)-specific extended short-chain dehydrogenase/reductase from Candida parapsilosis | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 123 | |
dc.date.issued | 2012 | |
local.identifier.absfor | 060199 - Biochemistry and Cell Biology not elsewhere classified | |
local.identifier.ariespublication | f5625xPUB6951 | |
local.type.status | Published Version | |
local.contributor.affiliation | Wang, Qiuyan, Hangzhou Normal University | |
local.contributor.affiliation | Shen, Linghong, Hangzhou Normal University | |
local.contributor.affiliation | Ye, Tingting, Hangzhou Normal University | |
local.contributor.affiliation | Cao, Dan, Hangzhou Normal University | |
local.contributor.affiliation | Chen, Rong, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Pei, Xiaolin, Hangzhou Normal University | |
local.contributor.affiliation | Xie, Tian, Hangzhou Normal University | |
local.contributor.affiliation | Li, Yan, Heihua Holdings Group | |
local.contributor.affiliation | Gong, Weibo, Meihua Holings Group | |
local.contributor.affiliation | Yen, Xiaopu, Hangzhou Normal University | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.startpage | 690 | |
local.bibliographicCitation.lastpage | 694 | |
local.identifier.doi | 10.1016/j.biortech.2012.07.060 | |
dc.date.updated | 2016-02-24T09:32:07Z | |
local.identifier.scopusID | 2-s2.0-84866763704 | |
local.identifier.thomsonID | 000310401100099 | |
Collections | ANU Research Publications |
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