Wang, Qiuyan; Shen, Linghong; Ye, Tingting; Cao, Dan; Chen, Rong; Pei, Xiaolin; Xie, Tian; Li, Yan; Gong, Weibo; Yen, Xiaopu
The gene encoding a putative protein from Candida parapsilosis CDC317 (CPE) was cloned and overexpressed in Escherichia coli. The protein was amenable to overexpression in E. coli and constituted 35% of the total cell protein content. The optimal activity was determined at pH 5.5 and 40°C with the substrate 4-chloro-3-oxobutanoate ethyl ester (COBE). The optical purity of the product was over 99% enantiomeric excess for the (S)-isomer, and the molar conversion yield of the product was 91.1%....[Show more]
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