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Specific Ion Effects: Role of Salt and Buffer in Protonation of Cytochrome c

Bostrom, Mathias Anders; Williams, David; Ninham, Barry

Description

Changes in background salt and buffer are known to influence the properties of proteins. The reasons have remained obscure. The challenge posed by many such problems is this. Can physical chemistry contribute any predictive quantitative insights to what is in effect the simplest macromolecular solution behavior? Or must all remain specific? Our thesis is that it can. For definiteness we consider here as an illustrative example: surface pH and protonation equilibria of cytochrome c. We...[Show more]

dc.contributor.authorBostrom, Mathias Anders
dc.contributor.authorWilliams, David
dc.contributor.authorNinham, Barry
dc.date.accessioned2015-12-13T22:40:06Z
dc.date.available2015-12-13T22:40:06Z
dc.identifier.issn1292-8941
dc.identifier.urihttp://hdl.handle.net/1885/78089
dc.description.abstractChanges in background salt and buffer are known to influence the properties of proteins. The reasons have remained obscure. The challenge posed by many such problems is this. Can physical chemistry contribute any predictive quantitative insights to what is in effect the simplest macromolecular solution behavior? Or must all remain specific? Our thesis is that it can. For definiteness we consider here as an illustrative example: surface pH and protonation equilibria of cytochrome c. We demonstrate an important role for ionic dispersion forces, missing from previous theoretical treatments. Unlike charge interactions these are different for each ionic species, and act between a protein and both salt and buffer ions. The charge of proteins depends not only on pH, ionic charge, and salt concentration. Taking ionic dispersion forces into account goes some way towards explaining the dependence on ionic species. We demonstrate why the addition of buffer can have profound effects, including reversal of the salt dependence of the protein charge.
dc.publisherSpringer
dc.sourceEuropean Physical Journal E
dc.subjectKeywords: Electrolytes; Ions; Molten materials; pH effects; Phase equilibria; Proteins; Salts; Ionic charge; Ionic dispersion; Molten salts; Structure of associated liquids; Pigments; buffer; cytochrome c; electrolyte; inorganic salt; ion; proton; water; article; c
dc.titleSpecific Ion Effects: Role of Salt and Buffer in Protonation of Cytochrome c
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume13
dc.date.issued2004
local.identifier.absfor020406 - Surfaces and Structural Properties of Condensed Matter
local.identifier.ariespublicationMigratedxPub6774
local.type.statusPublished Version
local.contributor.affiliationBostrom, Mathias Anders, Linkoping University
local.contributor.affiliationWilliams, David, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationNinham, Barry, College of Physical and Mathematical Sciences, ANU
local.bibliographicCitation.startpage239
local.bibliographicCitation.lastpage245
local.identifier.doi10.1140/epje/i2003-10072-0
dc.date.updated2015-12-11T09:54:02Z
local.identifier.scopusID2-s2.0-2642556412
CollectionsANU Research Publications

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