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Proteome reference maps of Medicago truncatula embryogenic cell cultures generated from single protoplasts

Imin, Nijat; De Jong, Femke; Mathesius, Ulrike; Van Noorden, Giel; Saeed, Nasir; Wang, Xin-Ding; Rose, Ray J; Rolfe, Barry


Using a combination of two-dimensional gel electrophoresis (2-DE) protein mapping and mass spectrometry (MS) analysis, we have established proteome reference maps of Medicago truncatula embryogenic tissue culture cells. The cultures were generated from single protoplasts, which provided a relatively homogenous cell population. We used these to analyze protein expression at the globular stages of somatic embryogenesis, which is the earliest morphogenetic embryonic stage. Over 3000 proteins could...[Show more]

CollectionsANU Research Publications
Date published: 2004
Type: Journal article
Source: Proteomics
DOI: 10.1002/pmic.200300803


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