Inhibition of Protein Interactions with the β 2 Sliding Clamp of Escherichia coli DNA Polymerase III by Peptides from β 2 -Binding Proteins
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Wijffels, Gene; Dalrymple, Brian Paul; Prosselkov, Pavel; Kongsuwan, Kritaya; Epa, V; Lilley, Penelope; Jergic, Slobodan; Buchardt, Jens; Brown, Susan Elizabeth; Alewood, Paul F; Jennings, Philip; Dixon, Nicholas
Description
The sliding clamp of the Escherichia coli replisome is now understood to interact with many proteins involved in DNA synthesis and repair. A universal interaction motif is proposed to be one mechanism by which those proteins bind the E. coli sliding clamp, a homodimer of the β subunit, at a single site on the dimer. The numerous β2-binding proteins have various versions of the consensus interaction motif, including a related hexameric sequence. To determine if the variants of the motif could...[Show more]
dc.contributor.author | Wijffels, Gene | |
---|---|---|
dc.contributor.author | Dalrymple, Brian Paul | |
dc.contributor.author | Prosselkov, Pavel | |
dc.contributor.author | Kongsuwan, Kritaya | |
dc.contributor.author | Epa, V | |
dc.contributor.author | Lilley, Penelope | |
dc.contributor.author | Jergic, Slobodan | |
dc.contributor.author | Buchardt, Jens | |
dc.contributor.author | Brown, Susan Elizabeth | |
dc.contributor.author | Alewood, Paul F | |
dc.contributor.author | Jennings, Philip | |
dc.contributor.author | Dixon, Nicholas | |
dc.date.accessioned | 2015-12-13T22:39:41Z | |
dc.date.available | 2015-12-13T22:39:41Z | |
dc.identifier.issn | 0006-2960 | |
dc.identifier.uri | http://hdl.handle.net/1885/77890 | |
dc.description.abstract | The sliding clamp of the Escherichia coli replisome is now understood to interact with many proteins involved in DNA synthesis and repair. A universal interaction motif is proposed to be one mechanism by which those proteins bind the E. coli sliding clamp, a homodimer of the β subunit, at a single site on the dimer. The numerous β2-binding proteins have various versions of the consensus interaction motif, including a related hexameric sequence. To determine if the variants of the motif could contribute to the competition of the β-binding proteins for the β2 site, synthetic peptides derived from the putative β2-binding motifs were assessed for their abilities to inhibit protein-β2 interactions, to bind directly to β2, and to inhibit DNA synthesis in vitro. A hierarchy emerged, which was consistent with sequence similarity to the pentameric consensus motif, QL(S/D)LF, and peptides containing proposed hexameric motifs were shown to have activities comparable to those containing the consensus sequence. The hierarchy of peptide binding may be indicative of a competitive hierarchy for the binding of proteins to β2 in various stages or circumstances of DNA replication and repair. | |
dc.publisher | American Chemical Society | |
dc.source | Biochemistry | |
dc.subject | Keywords: Dimers; DNA; Enzyme inhibition; Escherichia coli; Proteins; Synthesis (chemical); DNA replication; Hexameric motifs; Protein interactions; Synthetic peptides; Biochemistry; binding protein; dimer; DNA directed DNA polymerase alpha; peptide derivative; pro | |
dc.title | Inhibition of Protein Interactions with the β 2 Sliding Clamp of Escherichia coli DNA Polymerase III by Peptides from β 2 -Binding Proteins | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.description.refereed | Yes | |
local.identifier.citationvolume | 43 | |
dc.date.issued | 2004 | |
local.identifier.absfor | 030499 - Medicinal and Biomolecular Chemistry not elsewhere classified | |
local.identifier.ariespublication | MigratedxPub6634 | |
local.type.status | Published Version | |
local.contributor.affiliation | Wijffels, Gene, CSIRO Livestock | |
local.contributor.affiliation | Dalrymple, Brian Paul, CSIRO | |
local.contributor.affiliation | Prosselkov, Pavel, College of Physical and Mathematical Sciences, ANU | |
local.contributor.affiliation | Kongsuwan, Kritaya, CSIRO Livestock | |
local.contributor.affiliation | Epa, V, Institute of Molecular Bioscience | |
local.contributor.affiliation | Lilley, Penelope, College of Physical and Mathematical Sciences, ANU | |
local.contributor.affiliation | Jergic, Slobodan, College of Physical and Mathematical Sciences, ANU | |
local.contributor.affiliation | Buchardt, Jens, CSIRO | |
local.contributor.affiliation | Brown, Susan Elizabeth, College of Physical and Mathematical Sciences, ANU | |
local.contributor.affiliation | Alewood, Paul F, University of Queensland | |
local.contributor.affiliation | Jennings, Philip, College of Physical and Mathematical Sciences, ANU | |
local.contributor.affiliation | Dixon, Nicholas, College of Physical and Mathematical Sciences, ANU | |
local.bibliographicCitation.issue | 19 | |
local.bibliographicCitation.startpage | 5661 | |
local.bibliographicCitation.lastpage | 5671 | |
local.identifier.doi | 10.1021/bi036229j | |
dc.date.updated | 2015-12-11T09:51:46Z | |
local.identifier.scopusID | 2-s2.0-2442507194 | |
Collections | ANU Research Publications |
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