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Inhibition of Protein Interactions with the β 2 Sliding Clamp of Escherichia coli DNA Polymerase III by Peptides from β 2 -Binding Proteins

Wijffels, Gene; Dalrymple, Brian Paul; Prosselkov, Pavel; Kongsuwan, Kritaya; Epa, V; Lilley, Penelope; Jergic, Slobodan; Buchardt, Jens; Brown, Susan Elizabeth; Alewood, Paul F; Jennings, Philip; Dixon, Nicholas

Description

The sliding clamp of the Escherichia coli replisome is now understood to interact with many proteins involved in DNA synthesis and repair. A universal interaction motif is proposed to be one mechanism by which those proteins bind the E. coli sliding clamp, a homodimer of the β subunit, at a single site on the dimer. The numerous β2-binding proteins have various versions of the consensus interaction motif, including a related hexameric sequence. To determine if the variants of the motif could...[Show more]

dc.contributor.authorWijffels, Gene
dc.contributor.authorDalrymple, Brian Paul
dc.contributor.authorProsselkov, Pavel
dc.contributor.authorKongsuwan, Kritaya
dc.contributor.authorEpa, V
dc.contributor.authorLilley, Penelope
dc.contributor.authorJergic, Slobodan
dc.contributor.authorBuchardt, Jens
dc.contributor.authorBrown, Susan Elizabeth
dc.contributor.authorAlewood, Paul F
dc.contributor.authorJennings, Philip
dc.contributor.authorDixon, Nicholas
dc.date.accessioned2015-12-13T22:39:41Z
dc.date.available2015-12-13T22:39:41Z
dc.identifier.issn0006-2960
dc.identifier.urihttp://hdl.handle.net/1885/77890
dc.description.abstractThe sliding clamp of the Escherichia coli replisome is now understood to interact with many proteins involved in DNA synthesis and repair. A universal interaction motif is proposed to be one mechanism by which those proteins bind the E. coli sliding clamp, a homodimer of the β subunit, at a single site on the dimer. The numerous β2-binding proteins have various versions of the consensus interaction motif, including a related hexameric sequence. To determine if the variants of the motif could contribute to the competition of the β-binding proteins for the β2 site, synthetic peptides derived from the putative β2-binding motifs were assessed for their abilities to inhibit protein-β2 interactions, to bind directly to β2, and to inhibit DNA synthesis in vitro. A hierarchy emerged, which was consistent with sequence similarity to the pentameric consensus motif, QL(S/D)LF, and peptides containing proposed hexameric motifs were shown to have activities comparable to those containing the consensus sequence. The hierarchy of peptide binding may be indicative of a competitive hierarchy for the binding of proteins to β2 in various stages or circumstances of DNA replication and repair.
dc.publisherAmerican Chemical Society
dc.sourceBiochemistry
dc.subjectKeywords: Dimers; DNA; Enzyme inhibition; Escherichia coli; Proteins; Synthesis (chemical); DNA replication; Hexameric motifs; Protein interactions; Synthetic peptides; Biochemistry; binding protein; dimer; DNA directed DNA polymerase alpha; peptide derivative; pro
dc.titleInhibition of Protein Interactions with the β 2 Sliding Clamp of Escherichia coli DNA Polymerase III by Peptides from β 2 -Binding Proteins
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume43
dc.date.issued2004
local.identifier.absfor030499 - Medicinal and Biomolecular Chemistry not elsewhere classified
local.identifier.ariespublicationMigratedxPub6634
local.type.statusPublished Version
local.contributor.affiliationWijffels, Gene, CSIRO Livestock
local.contributor.affiliationDalrymple, Brian Paul, CSIRO
local.contributor.affiliationProsselkov, Pavel, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationKongsuwan, Kritaya, CSIRO Livestock
local.contributor.affiliationEpa, V, Institute of Molecular Bioscience
local.contributor.affiliationLilley, Penelope, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationJergic, Slobodan, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationBuchardt, Jens, CSIRO
local.contributor.affiliationBrown, Susan Elizabeth, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationAlewood, Paul F, University of Queensland
local.contributor.affiliationJennings, Philip, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationDixon, Nicholas, College of Physical and Mathematical Sciences, ANU
local.bibliographicCitation.issue19
local.bibliographicCitation.startpage5661
local.bibliographicCitation.lastpage5671
local.identifier.doi10.1021/bi036229j
dc.date.updated2015-12-11T09:51:46Z
local.identifier.scopusID2-s2.0-2442507194
CollectionsANU Research Publications

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