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Universal fast walking appiled to cDNA

Park, Daniel J; Renfree, Marilyn B; Graves, Jennifer

Description

The elucidation of cDNA sequence remains problematic in cases such as genes possessing long coding regions, low expression levels, or poor library coverage. The recently described Universal Fast Walk (UFW) procedure offers a means of determining DNA sequence adjacent to characterised regions. To date, however, the approach has been applied only to genomic DNA. We demonstrate the first successful application of the UFW procedure to the elucidation of cDNA sequence, a previously unknown region of...[Show more]

CollectionsANU Research Publications
Date published: 2004
Type: Journal article
URI: http://hdl.handle.net/1885/77806
Source: Preparative Biochemistry anf Biotechnology
DOI: 10.1081/PB-120030871

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