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Quantitative Forster resonance energy transfer efficiency measurements using simultaneous spectral unmixing of excitation and emission spectra

Mustafa, Sanam; Hannagan, John; Rigby, Paul; Pfleger, Kevin D. G.; Corry, Ben


Accurate quantification of Förster resonance energy transfer (FRET) using intensity-based methods is difficult due to the overlap of fluorophore excitation and emission spectra. Consequently, mechanisms are required to remove bleedthrough of the donor em

CollectionsANU Research Publications
Date published: 2013
Type: Journal article
Source: Journal of Biomedical Optics
DOI: 10.1117/1.JBO.18.2.026024


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