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Efficacy of DNA and Fowlpox virus priming/boosting vaccines for Simian/Human immunodeficiency virus

Dale, C Jane; De Rose, Robert; Stratov, Ivan; Chea, Socheata; Montefiori, David C; Thomson, Scott; Ramshaw, Ian; Coupar, Barbara E H; Boyle, David B; Law, Matthew G; Kent, Stephen J

Description

Further advances are required in understanding protection from AIDS by T-cell immunity. We analyzed a set of multigenic simian/human immunodeficiency virus (SHIV) DNA and fowlpox virus priming and boosting vaccines for immunogenicity and protective efficacy in outbred pigtail macaques. The number of vaccinations required, the effect of DNA vaccination alone, and the effect of cytokine (gamma interferon) coexpression by the fowlpox virus boost was also studied. A coordinated induction of high...[Show more]

dc.contributor.authorDale, C Jane
dc.contributor.authorDe Rose, Robert
dc.contributor.authorStratov, Ivan
dc.contributor.authorChea, Socheata
dc.contributor.authorMontefiori, David C
dc.contributor.authorThomson, Scott
dc.contributor.authorRamshaw, Ian
dc.contributor.authorCoupar, Barbara E H
dc.contributor.authorBoyle, David B
dc.contributor.authorLaw, Matthew G
dc.contributor.authorKent, Stephen J
dc.date.accessioned2015-12-13T22:39:06Z
dc.date.available2015-12-13T22:39:06Z
dc.identifier.issn0022-538X
dc.identifier.urihttp://hdl.handle.net/1885/77639
dc.description.abstractFurther advances are required in understanding protection from AIDS by T-cell immunity. We analyzed a set of multigenic simian/human immunodeficiency virus (SHIV) DNA and fowlpox virus priming and boosting vaccines for immunogenicity and protective efficacy in outbred pigtail macaques. The number of vaccinations required, the effect of DNA vaccination alone, and the effect of cytokine (gamma interferon) coexpression by the fowlpox virus boost was also studied. A coordinated induction of high levels of broadly reactive CD4 and CD8 T-cell immune responses was induced by sequential DNA and fowlpox virus vaccination. The immunogenicity of regimens utilizing fowlpox virus coexpressing gamma interferon, a single DNA priming vaccination, or DNA vaccines alone was inferior. Significant control of a virulent SHIV challenge was observed despite a loss of SHIV-specific proliferating T cells. The outcome of challenge with virulent SHIVmn229 correlated with vaccine immunogenicity except that DNA vaccination alone primed for protection almost as effectively as the DNA/ fowlpox virus regimen despite negligible immunogenicity by standard assays. These studies suggest that priming of immunity with DNA and fowlpox virus vaccines could delay AIDS in humans.
dc.publisherAmerican Society for Microbiology
dc.sourceJournal of Virology
dc.subjectKeywords: CD4 antigen; CD8 antigen; cytokine; DNA vaccine; gamma interferon; virus vaccine; acquired immune deficiency syndrome; animal cell; article; assay; controlled study; DNA virus; drug efficacy; Fowlpox virus; gene induction; Human immunodeficiency virus; im
dc.titleEfficacy of DNA and Fowlpox virus priming/boosting vaccines for Simian/Human immunodeficiency virus
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume78
dc.date.issued2004
local.identifier.absfor110799 - Immunology not elsewhere classified
local.identifier.ariespublicationMigratedxPub6461
local.type.statusPublished Version
local.contributor.affiliationDale, C Jane, University of Melbourne
local.contributor.affiliationDe Rose, Robert, University of Melbourne
local.contributor.affiliationStratov, Ivan, University of Melbourne
local.contributor.affiliationChea, Socheata, University of Melbourne
local.contributor.affiliationMontefiori, David C, Duke University
local.contributor.affiliationThomson, Scott, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationRamshaw, Ian, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationCoupar, Barbara E H, Duke University
local.contributor.affiliationBoyle, David B, CSIRO Division of Livestock Industries
local.contributor.affiliationLaw, Matthew G, University of New South Wales
local.contributor.affiliationKent, Stephen J, University of Melbourne
local.bibliographicCitation.issue24
local.bibliographicCitation.startpage13819
local.bibliographicCitation.lastpage13828
local.identifier.doi10.1128/JVI.78.24.13819-13828.2004
dc.date.updated2015-12-11T09:46:46Z
local.identifier.scopusID2-s2.0-10044271240
CollectionsANU Research Publications

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