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The behavior of R-ovalbumin and its individual components A(1), A(2), and A(3) in urea solution: Kinetics and equilibria

McKenzie, Hugh; Frier, Robert


Procedures are described for the isolation of the individual components A1, A2, and A3 of native R-ovalbumin from freshly laid domestic hen eggs. Because heavy metal ion contaminants result in spurious irreproducible kinetics, particularly at high pH, considerable care is taken to avoid their presence. Kinetics studies are made of the behavior of whole R-ovalbumin and its individual components in urea solution over the pH range 3.7-9.6 following the reaction by determining absorbance...[Show more]

CollectionsANU Research Publications
Date published: 2003
Type: Journal article
Source: Journal of Protein Chemistry
DOI: 10.1023/A:1025076621515


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