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Opposing effects of reduced DNA methylation on flowering time in Arabidopsis thaliana

Genger, Ruth; Peacock, W James; Dennis, Elizabeth; Finnegan, E. Jean

Description

Demethylation of DNA promotes flowering in plants from the vernalization-responsive ecotype C24 of Arabidopsis thaliana (L.) Heynh., but delays flowering in the ecotype Landsberg erecta which is not responsive to vernalization. To investigate these contrasting effects of low methylation we have monitored flowering times and expression of two repressors of flowering, FLC and FWA, in low-methylation plants from three late-flowering mutants in the ecotype Landsberg erecta. Demethylation of DNA...[Show more]

dc.contributor.authorGenger, Ruth
dc.contributor.authorPeacock, W James
dc.contributor.authorDennis, Elizabeth
dc.contributor.authorFinnegan, E. Jean
dc.date.accessioned2015-12-13T22:37:28Z
dc.date.available2015-12-13T22:37:28Z
dc.identifier.issn0032-0935
dc.identifier.urihttp://hdl.handle.net/1885/77115
dc.description.abstractDemethylation of DNA promotes flowering in plants from the vernalization-responsive ecotype C24 of Arabidopsis thaliana (L.) Heynh., but delays flowering in the ecotype Landsberg erecta which is not responsive to vernalization. To investigate these contrasting effects of low methylation we have monitored flowering times and expression of two repressors of flowering, FLC and FWA, in low-methylation plants from three late-flowering mutants in the ecotype Landsberg erecta. Demethylation of DNA decreased FLC expression in the vernalization-responsive mutants, but was not associated with a promotion of flowering; rather, in some lines, demethylation delayed flowering. The opposing effects of demethylation could be explained by its differential effect on the expression of two repressors of flowering. FLC was down-regulated in plants with low methylation, promoting flowering, while FWA was activated in response to demethylation, which probably delays the transition to flowering. Expression of the FWA gene did not delay flowering in plants of ecotype C24; our data suggest that the FWA protein of C24 may be nonfunctional.
dc.publisherSpringer
dc.sourcePlanta
dc.subjectKeywords: Data reduction; DNA; Proteins; Demethylation; Plants (botany); Arabidopsis; Arabidopsis thaliana; Embryophyta; Arabidopsis protein; FLF protein, Arabidopsis; FWA protein, Arabidopsis; homeodomain protein; MADS domain protein; transcription factor; vegetab Arabidopsis; Epigenetics; FLOWERING LOCUS C; FWA; METHYLTRANSFERASEI antisense; Vernalization
dc.titleOpposing effects of reduced DNA methylation on flowering time in Arabidopsis thaliana
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume216
dc.date.issued2003
local.identifier.absfor060799 - Plant Biology not elsewhere classified
local.identifier.ariespublicationMigratedxPub5994
local.type.statusPublished Version
local.contributor.affiliationGenger, Ruth, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationPeacock, W James, CSIRO Division of Plant Industry
local.contributor.affiliationDennis, Elizabeth, CSIRO
local.contributor.affiliationFinnegan, E. Jean, CSIRO
local.bibliographicCitation.issue3
local.bibliographicCitation.startpage461
local.bibliographicCitation.lastpage466
dc.date.updated2015-12-11T09:36:53Z
local.identifier.scopusID2-s2.0-0037837466
CollectionsANU Research Publications

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