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Simultaneous time resolution of the emission spectra of fluorescent proteins and zooxanthellar chlorophyll in reef building corals

Gilmore, Adam; Larkum, A; Salih, A; Itoh, Shigeru; Shibata, Y; Bena, C; Yamasaki, H; Papina, M; van Woesik, R

Description

Light is absorbed by photosynthetic algal symbionts (i.e. zooxanthellae) and by chromophoric fluorescent proteins (FP) in reef-building coral tissue. We used a streak-camera spectrograph equipped with a pulsed, blue laser diode (50 ps, 405 nm) to simultaneously resolve the fluorescence spectra and kinetics for both the FP and the zooxanthellae. Shallow water (<9 m)-dwelling Acropora spp. and Plesiastrea versipora specimens were collected from Okinawa, Japan, and Sydney, Australia, respectively....[Show more]

dc.contributor.authorGilmore, Adam
dc.contributor.authorLarkum, A
dc.contributor.authorSalih, A
dc.contributor.authorItoh, Shigeru
dc.contributor.authorShibata, Y
dc.contributor.authorBena, C
dc.contributor.authorYamasaki, H
dc.contributor.authorPapina, M
dc.contributor.authorvan Woesik, R
dc.date.accessioned2015-12-13T22:37:21Z
dc.date.available2015-12-13T22:37:21Z
dc.identifier.issn0031-8655
dc.identifier.urihttp://hdl.handle.net/1885/77063
dc.description.abstractLight is absorbed by photosynthetic algal symbionts (i.e. zooxanthellae) and by chromophoric fluorescent proteins (FP) in reef-building coral tissue. We used a streak-camera spectrograph equipped with a pulsed, blue laser diode (50 ps, 405 nm) to simultaneously resolve the fluorescence spectra and kinetics for both the FP and the zooxanthellae. Shallow water (<9 m)-dwelling Acropora spp. and Plesiastrea versipora specimens were collected from Okinawa, Japan, and Sydney, Australia, respectively. The main FP emitted light in the blue, blue-green and green emission regions with each species exhibiting distinct color morphs and spectra. All corals showed rapidly decaying species and reciprocal rises in greener emission components indicating Förster resonance energy transfer (FRET) between FP populations. The energy transfer modes were around 250 ps, and the main decay modes of the acceptor FP were typically 1900-2800 ps. All zooxanthellae emitted similar spectra and kinetics with peak emission (∼683 nm) mainly from photosystem II (PSII) chlorophyll (chl) a. Compared with the FP, the PSII emission exhibited similar rise times but much faster decay times, typically around 640-760 ps. The fluorescence kinetics and excitation versus emission mapping indicated that the FP emission played only a minor role, if any, in chl excitation. We thus suggest the FP could only indirectly act to absorb, screen and scatter light to protect PSII and underlying and surrounding animal tissue from excess visible and UV light. We conclude that our time-resolved spectral analysis and simulation revealed new FP emission components that would not be easily resolved at steady state because of their relatively rapid decays due to efficient FRET. We believe the methods show promise for future studies of coral bleaching and for potentially identifying FP species for use as genetic markers and FRET partners, like the related green FP from Aequorea spp.
dc.publisherAmerican Society of Photobiology
dc.sourcePhotochemistry and Photobiology
dc.subjectKeywords: chlorophyll; chlorophyll a; fluorescent protein; protein; unclassified drug; photoprotein; alga; analytic method; conference paper; energy transfer; fluorescence spectroscopy; forster resonance energy transfer; kinetics; light scattering; nonhuman; photos
dc.titleSimultaneous time resolution of the emission spectra of fluorescent proteins and zooxanthellar chlorophyll in reef building corals
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume77
dc.date.issued2003
local.identifier.absfor060602 - Animal Physiology - Cell
local.identifier.ariespublicationMigratedxPub5921
local.type.statusPublished Version
local.contributor.affiliationGilmore, Adam, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationLarkum, A, University of Sydney
local.contributor.affiliationSalih, A, University of Sydney
local.contributor.affiliationItoh, Shigeru, Nagoya University
local.contributor.affiliationShibata, Y, Nagoya University
local.contributor.affiliationBena, C, University of the Ryukyus
local.contributor.affiliationYamasaki, H, University of the Ryukyus
local.contributor.affiliationPapina, M, Russian Academy of Sciences
local.contributor.affiliationvan Woesik, R, Florida Institute of Technology
local.bibliographicCitation.issue5
local.bibliographicCitation.startpage515
local.bibliographicCitation.lastpage523
local.identifier.doi10.1562/0031-8655(2003)077<0515:STROTE>2.0.CO;2
dc.date.updated2015-12-11T09:36:22Z
local.identifier.scopusID2-s2.0-0037928115
CollectionsANU Research Publications

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