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Age-related pseudocapillarization of the human liver

McLean, Allan J; Cogger, Victoria; Chong, Guan; Warren, Alessandra; Markus, Astrid; Dahlstrom, Jane; Le Couteur, David

Description

Age-related changes in liver function are important because they may promote susceptibility to adverse drug reactions, neurotoxicity, atherosclerosis, and other important diseases in older people. Age-related changes in the rat hepatic sinusoidal endothelium, termed pseudocapillarization, have been described recently and these may contribute to hepatic impairment. The present study has examined surgical and post-mortem specimens with immunohistochemistry and transmission electron microscopy to...[Show more]

dc.contributor.authorMcLean, Allan J
dc.contributor.authorCogger, Victoria
dc.contributor.authorChong, Guan
dc.contributor.authorWarren, Alessandra
dc.contributor.authorMarkus, Astrid
dc.contributor.authorDahlstrom, Jane
dc.contributor.authorLe Couteur, David
dc.date.accessioned2015-12-13T22:36:58Z
dc.date.available2015-12-13T22:36:58Z
dc.identifier.issn0022-3417
dc.identifier.urihttp://hdl.handle.net/1885/77028
dc.description.abstractAge-related changes in liver function are important because they may promote susceptibility to adverse drug reactions, neurotoxicity, atherosclerosis, and other important diseases in older people. Age-related changes in the rat hepatic sinusoidal endothelium, termed pseudocapillarization, have been described recently and these may contribute to hepatic impairment. The present study has examined surgical and post-mortem specimens with immunohistochemistry and transmission electron microscopy to determine whether pseudocapillarization also occurs in older humans. The age of the subject, independent of systemic disease or hepatic pathology in surgical and post-mortem samples of human liver, was associated with increased peri-sinusoidal expression of von Willebrand's factor, collagen I, collagen IV, and staining with Masson's trichrome. Electron microscopy revealed significant age-related thickening of the sinusoidal endothelium (young 165 ± 17 nm, middle age 222 ± 11 nm, older 289 ± 9 nm, p < 0.001) with loss of fenestrations (young 7.7 ± 0.7 per 10 μm, middle age 3.6 ± 0.5 per 10 μm, older 1.5 ± 0.4 per 10 μm, p < 0.001), and age-related deposition of basal lamina and collagen. In conclusion, ageing in humans is associated with morphological changes in the sinusoidal endothelium and space of Disse which are presumptively related to the ageing process and potentially represent an important link between the ageing process and disease susceptibility.
dc.publisherJohn Wiley & Sons Inc
dc.sourceJournal of Pathology
dc.subjectKeywords: collagen type 1; collagen type 4; von Willebrand factor; adolescent; adult; adverse drug reaction; age distribution; aged; aging; article; atherosclerosis; autopsy; basement membrane; child; controlled study; disease predisposition; human; human tissue; i Ageing; Defenestration; Electron microscopy; Hepatic sinusoid; Immunohistochemistry; Liver; Pseudocapillarization
dc.titleAge-related pseudocapillarization of the human liver
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume200
dc.date.issued2003
local.identifier.absfor111603 - Systems Physiology
local.identifier.ariespublicationMigratedxPub5875
local.type.statusPublished Version
local.contributor.affiliationMcLean, Allan J, Canberra Hospital
local.contributor.affiliationCogger, Victoria, University of Sydney
local.contributor.affiliationChong, Guan, University of Sydney
local.contributor.affiliationWarren, Alessandra, University of Sydney
local.contributor.affiliationMarkus, Astrid, University of Melbourne
local.contributor.affiliationDahlstrom, Jane, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationLe Couteur, David, University of Sydney
local.bibliographicCitation.startpage112
local.bibliographicCitation.lastpage117
local.identifier.doi10.1002/path.1328
dc.date.updated2015-12-11T09:34:27Z
local.identifier.scopusID2-s2.0-0037986813
CollectionsANU Research Publications

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