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Cone Visual Pigments of the Australian Marsupials, the Stripe-Faced (Sminthopsis macroura) and Fat-Tailed (Sminthopsis crassicaudata) Dunnarts: Sequence and Inferred Spectral Properties

Strachan, Jessica; Chang, Ling Yu; Wakefield, Matthew; Graves, Jennifer; Deeb, Samir

Description

Studies of color vision in marsupial mammals have been very limited. Two photoreceptor genes have been characterized from the tammar wallaby, but a third cone pigment was suggested by microspectrophotometric measurements on cone photoreceptors in two other species, including the fat-tailed dunnart, Sminthopsis crassicaudata. To determine the sequence and infer absorption maxima of the cone photoreceptor pigments of S. crassicaudata and the related stripe-faced dunnart (Sminthopsis macroura), we...[Show more]

dc.contributor.authorStrachan, Jessica
dc.contributor.authorChang, Ling Yu
dc.contributor.authorWakefield, Matthew
dc.contributor.authorGraves, Jennifer
dc.contributor.authorDeeb, Samir
dc.date.accessioned2015-12-13T22:36:31Z
dc.date.available2015-12-13T22:36:31Z
dc.identifier.issn0952-5238
dc.identifier.urihttp://hdl.handle.net/1885/76800
dc.description.abstractStudies of color vision in marsupial mammals have been very limited. Two photoreceptor genes have been characterized from the tammar wallaby, but a third cone pigment was suggested by microspectrophotometric measurements on cone photoreceptors in two other species, including the fat-tailed dunnart, Sminthopsis crassicaudata. To determine the sequence and infer absorption maxima of the cone photoreceptor pigments of S. crassicaudata and the related stripe-faced dunnart (Sminthopsis macroura), we have used evolutionarily conserved sequences of the cone pigments of other species, including the tammar wallaby, to design primers to amplify the S. macroura and S. crassicaudata pigment sequences by the polymerase chain reaction (PCR) using genomic DNA or retinal cDNA as a template. These primers will be useful for amplifying cone opsin coding sequences from a variety of vertebrates. Amplified products were directly sequenced to determine gene structure and coding sequences. The inferred amino acid sequences of the cone visual pigments indicated that both species have middle-wave-sensitive (MWS) pigments with a predicted absorption maximum (λmax) at 530 nm, and ultraviolet-sensitive (UVS) pigments with a predicted λmax at 360 nm. The MWS pigments of the two species differ by two, and UVS by three amino acid positions. No evidence was obtained for a third cone pigment in either species.
dc.publisherCambridge University Press
dc.sourceVisual Neuroscience
dc.subjectKeywords: complementary DNA; genomic DNA; opsin; visual pigment; amino acid sequence; animal tissue; Australia; color vision; conference paper; dunnart; female; gene amplification; gene structure; genetic code; genetic conservation; human; marsupial; microspectroph Cone opsins; Dunnart; Marsupials; Spectral tuning
dc.titleCone Visual Pigments of the Australian Marsupials, the Stripe-Faced (Sminthopsis macroura) and Fat-Tailed (Sminthopsis crassicaudata) Dunnarts: Sequence and Inferred Spectral Properties
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume21
dc.date.issued2004
local.identifier.absfor060409 - Molecular Evolution
local.identifier.ariespublicationMigratedxPub5592
local.type.statusPublished Version
local.contributor.affiliationStrachan, Jessica, University of Washington
local.contributor.affiliationChang, Ling Yu, Washington University
local.contributor.affiliationWakefield, Matthew, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationGraves, Jennifer, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationDeeb, Samir, University of Washington
local.bibliographicCitation.issue3
local.bibliographicCitation.startpage223
local.bibliographicCitation.lastpage229
local.identifier.doi10.1017/S0952523804213281
dc.date.updated2016-02-24T09:48:45Z
local.identifier.scopusID2-s2.0-8344245296
CollectionsANU Research Publications

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