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PAT-seq: A method to study the integration of 3'-UTR dynamics with gene expression in the eukaryotic transcriptome

Harrison, Paul F.; Powell, David R.; Clancy, Jennifer; Preiss, Thomas; Boag, Peter R.; Traven, Ana; Seemann, Torsten; Beilharz, Traude H.


A major objective of systems biology is to quantitatively integrate multiple parameters from genome-wide measurements. To integrate gene expression with dynamics in poly(A) tail length and adenylation site, we developed a targeted next-generation sequencing approach, Poly(A)-Test RNA-sequencing. PAT-seq returns (i) digital gene expression, (ii) polyadenylation site/s, and (iii) the polyadenylation-state within and between eukaryotic transcriptomes. PAT-seq differs from previous 3′ focused...[Show more]

CollectionsANU Research Publications
Date published: 2015
Type: Journal article
Source: RNA
DOI: 10.1261/rna.048355.114


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