Skip navigation
Skip navigation

PAT-seq: A method to study the integration of 3'-UTR dynamics with gene expression in the eukaryotic transcriptome

Harrison, Paul F.; Powell, David R.; Clancy, Jennifer; Preiss, Thomas; Boag, Peter R.; Traven, Ana; Seemann, Torsten; Beilharz, Traude H.

Description

A major objective of systems biology is to quantitatively integrate multiple parameters from genome-wide measurements. To integrate gene expression with dynamics in poly(A) tail length and adenylation site, we developed a targeted next-generation sequencing approach, Poly(A)-Test RNA-sequencing. PAT-seq returns (i) digital gene expression, (ii) polyadenylation site/s, and (iii) the polyadenylation-state within and between eukaryotic transcriptomes. PAT-seq differs from previous 3′ focused...[Show more]

CollectionsANU Research Publications
Date published: 2015
Type: Journal article
URI: http://hdl.handle.net/1885/76766
Source: RNA
DOI: 10.1261/rna.048355.114

Download

File Description SizeFormat Image
01_Harrison_PAT-seq:_A_method_to_study_the_2015.pdf2.15 MBAdobe PDF    Request a copy


Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  20 July 2017/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator