Nitrogen-regulated hypermutator strain of Synechococcus sp. for use in in vivo artificial evolution
Artificially evolved variants of proteins with roles in photosynthesis may be selected most conveniently by using a photosynthetic organism, such as a cyanobacterium, whose growth depends on the function of the target protein. However, the limited transformation efficiency of even the most transformable cyanobacteria wastes much of the diversity of mutant libraries of genes produced in vitro, impairing the coverage of sequence space. This highlights the advantages of an in vivo approach for...[Show more]
|Collections||ANU Research Publications|
|Source:||Applied and Environmental Microbiology|
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