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Increased expression of a bacterial phosphotriesterase in Escherichia coli through directed evolution

Yu McLoughlin, Sean; Jackson, Colin; Liu, Jian-Wei; Ollis, David

Description

We devised a growth-based strategy for screening phosphotriesterase mutant libraries for variants with enhanced activity towards organophosphates that generate dimethyl phosphate when hydrolysed. Phosphotriesterase mutants were screened for activity by growing transformed Escherichia coli on agar plates containing methyl paraoxon as a sole phosphorus source. E. coli is capable of growth under these conditions when coexpressing the phosphotriesterase from Agrobacterium radiobacter P230 (OpdA)...[Show more]

CollectionsANU Research Publications
Date published: 2005
Type: Journal article
URI: http://hdl.handle.net/1885/76094
Source: Protein Expression and Purification
DOI: 10.1016/j.pep.2005.01.012

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