Purification, crystallization and preliminary crystallographic analysis of DehIVa, a dehalogenase from Burkholderia cepacia MBA4
Schmidberger, Jason; Oakley, Aaron; Tsang, Jimmy; Wilce, Matthew
Description
DehIVa is one of two dehalogenases produced by the soil- and water-borne bacterium Burkholderia cepacia MBA4. It acts to break down short-chain halogenated aliphatic acids through a nucleophilic attack and subsequent hydrolysis of an enzyme-substrate intermediate to remove the halide ions from L-enantiomers substituted at the C2 position (e.g L-2-monochloropropionic acid). Dehalogenases are an important group of enzymes that are responsible for breaking down a diverse range of halogenated...[Show more]
Collections | ANU Research Publications |
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Date published: | 2005 |
Type: | Journal article |
URI: | http://hdl.handle.net/1885/76092 |
Source: | Acta Crystallographica Section F: Structural Biology and Crystallization Communications |
DOI: | 10.1107/S1744309105002472 |
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01_Schmidberger_Purification,_crystallization_2005.pdf | 230.79 kB | Adobe PDF | Request a copy |
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