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Purification, crystallization and preliminary crystallographic analysis of DehIVa, a dehalogenase from Burkholderia cepacia MBA4

Schmidberger, Jason; Oakley, Aaron; Tsang, Jimmy; Wilce, Matthew


DehIVa is one of two dehalogenases produced by the soil- and water-borne bacterium Burkholderia cepacia MBA4. It acts to break down short-chain halogenated aliphatic acids through a nucleophilic attack and subsequent hydrolysis of an enzyme-substrate intermediate to remove the halide ions from L-enantiomers substituted at the C2 position (e.g L-2-monochloropropionic acid). Dehalogenases are an important group of enzymes that are responsible for breaking down a diverse range of halogenated...[Show more]

CollectionsANU Research Publications
Date published: 2005
Type: Journal article
Source: Acta Crystallographica Section F: Structural Biology and Crystallization Communications
DOI: 10.1107/S1744309105002472


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