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Plastid transformation for Rubisco engineering and protocols for assessing expression.

Whitney, Spencer; Sharwood, Robert

Description

The assimilation of CO2 within chloroplasts is catalyzed by the bi-functional enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase, Rubisco. Within higher plants the Rubisco large subunit gene, rbcL, is encoded in the plastid genome, while the Rubisco small subunit gene, RbcS is coded in the nucleus by a multi-gene family. Rubisco is considered a poor catalyst due to its slow turnover rate and its additional fixation of O2 that can result in wasteful loss of carbon through the energy...[Show more]

CollectionsANU Research Publications
Date published: 2014
Type: Book chapter
URI: http://hdl.handle.net/1885/76048
DOI: 10.1007/978-1-62703-995-6_15

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