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In vivo assessment of grafted cortical neural progenitor cells and host response to functionalized self-assembling peptide hydrogels and the implications for tissue repair

Rodriguez, Alexandra; Wang, Ting-Yi; Bruggeman, Kiara; Horgan, Conor; Li, R; Williams, Richard J; Parish, Clare L.; Nisbet, David

Description

Tissue specific scaffolds formed from minimalist N-fluorenylmethyloxycarbonyl self-assembling peptides (Fmoc-SAPs) have emerged as promising biomaterials due to their ease of synthesis and capacity to self-assemble via simple, non-covalent interactions into complex nanofibrous hydrogels. However, concerns remain over their biocompatibility and cytotoxicity for in vivo applications. Here, we demonstrate that these Fmoc-SAPs are biocompatible in vivo and well suited as a delivery vehicle for cell...[Show more]

dc.contributor.authorRodriguez, Alexandra
dc.contributor.authorWang, Ting-Yi
dc.contributor.authorBruggeman, Kiara
dc.contributor.authorHorgan, Conor
dc.contributor.authorLi, R
dc.contributor.authorWilliams, Richard J
dc.contributor.authorParish, Clare L.
dc.contributor.authorNisbet, David
dc.date.accessioned2015-12-13T22:32:56Z
dc.identifier.issn2050-750X
dc.identifier.urihttp://hdl.handle.net/1885/75797
dc.description.abstractTissue specific scaffolds formed from minimalist N-fluorenylmethyloxycarbonyl self-assembling peptides (Fmoc-SAPs) have emerged as promising biomaterials due to their ease of synthesis and capacity to self-assemble via simple, non-covalent interactions into complex nanofibrous hydrogels. However, concerns remain over their biocompatibility and cytotoxicity for in vivo applications. Here, we demonstrate that these Fmoc-SAPs are biocompatible in vivo and well suited as a delivery vehicle for cell transplantation. In order to determine the effect of tissue specific parameters, we designed three Fmoc-SAPs containing varying bioactive peptide sequences derived from extracellular matrix proteins, laminin and fibronectin. Fmoc-SAPs delivering cortical neural progenitor cells into the mouse brain display a limited foreign body response, effective functionalization and low cytotoxicity for at least 28 days. These results highlight the suitability of Fmoc-SAPs for improved neural tissue repair through the support of grafted cells and adjacent host parenchyma. Overall, we illustrate that Fmoc-SAPs are easily engineered materials for use as a tool in cell transplantation, where biocompatibility is key to promoting cell survival, enhancing the graft-host interface and attenuation of the inflammatory response for improved tissue repair outcomes.
dc.publisherRoyal Society of Chemistry
dc.sourceJournal of Materials Chemistry B
dc.titleIn vivo assessment of grafted cortical neural progenitor cells and host response to functionalized self-assembling peptide hydrogels and the implications for tissue repair
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume2
dc.date.issued2014
local.identifier.absfor090605 - Photodetectors, Optical Sensors and Solar Cells
local.identifier.ariespublicationU3488905xPUB4795
local.type.statusPublished Version
local.contributor.affiliationRodriguez, Alexandra, College of Engineering and Computer Science, ANU
local.contributor.affiliationWang, Ting-Yi, Florey Institute of Neuroscience & Mental Health, The University of Melbourne
local.contributor.affiliationBruggeman, Kiara, College of Engineering and Computer Science, ANU
local.contributor.affiliationHorgan, Conor, College of Engineering and Computer Science, ANU
local.contributor.affiliationLi, R, Centre for Chemistry and Biotechnology
local.contributor.affiliationWilliams, Richard J, Deakin University
local.contributor.affiliationParish, Clare L., University of Melbourne
local.contributor.affiliationNisbet, David, College of Engineering and Computer Science, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.issue44
local.bibliographicCitation.startpage7771
local.bibliographicCitation.lastpage7778
local.identifier.doi10.1039/c4tb01391c
local.identifier.absseo850504 - Solar-Photovoltaic Energy
dc.date.updated2015-12-11T09:12:00Z
local.identifier.scopusID2-s2.0-84908144090
local.identifier.thomsonID000344329200015
CollectionsANU Research Publications

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