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Characterization of Barmah Forest virus pathogenesis in a mouse model

Herrero, Lara; Lidbury, Brett; Bettadapura, Jayaram; Peng, Jian; Herrig, Belinda L; Hey-Cunningham, William J.; Sheng, Kuo-Ching; Zakhary, Andrew; Mahalingam, Suresh

Description

Alphaviruses including Barmah Forest virus (BFV) and Ross River virus (RRV) cause arthritis, arthralgia and myalgia in humans. The rheumatic symptoms in human BFV infection are very similar to those of RRV. Although RRV disease has been studied extensively, little is known about the pathogenesis of BFV infection. We sought to establish a mouse model for BFV to facilitate our understanding of BFV infectivity, tropism and pathogenesis, and to identify key pathological and immunological mechanisms...[Show more]

dc.contributor.authorHerrero, Lara
dc.contributor.authorLidbury, Brett
dc.contributor.authorBettadapura, Jayaram
dc.contributor.authorPeng, Jian
dc.contributor.authorHerrig, Belinda L
dc.contributor.authorHey-Cunningham, William J.
dc.contributor.authorSheng, Kuo-Ching
dc.contributor.authorZakhary, Andrew
dc.contributor.authorMahalingam, Suresh
dc.date.accessioned2015-12-13T22:32:10Z
dc.identifier.issn0022-1317
dc.identifier.urihttp://hdl.handle.net/1885/75454
dc.description.abstractAlphaviruses including Barmah Forest virus (BFV) and Ross River virus (RRV) cause arthritis, arthralgia and myalgia in humans. The rheumatic symptoms in human BFV infection are very similar to those of RRV. Although RRV disease has been studied extensively, little is known about the pathogenesis of BFV infection. We sought to establish a mouse model for BFV to facilitate our understanding of BFV infectivity, tropism and pathogenesis, and to identify key pathological and immunological mechanisms of BFV infection that may distinguish between infections with BFV and RRV. Here, to the best of our knowledge, we report the first study assessing the virulence and replication of several BFV isolates in a mouse model. We infected newborn Swiss outbred mice with BFV and established that the BFV2193 prototype was the most virulent strain. BFV2193 infection resulted in the highest mortality among all BFV variant isolates, comparable to that of RRV. In comparison with RRV, C57BL/6 mice infected with BFV showed delayed onset, moderate disease scores and early recovery of the disease. BFV replicated poorly in muscle and did not cause the severe myositis seen in RRV-infected mice. The mRNAs for the inflammatory mediators TNF-α, IL-6, CCL2 and arginase-1 were highly upregulated in RRV- but not BFV-infected muscle. To our knowledge, this is the first report of a mouse model of BFV infection, which we have used to demonstrate differences between BFV and RRV infections and to further understand disease pathogenesis. With an increasing number of BFV cases occurring annually, a better understanding of the disease mechanisms is essential for future therapeutic development.
dc.publisherSociety for General Microbiology
dc.sourceJournal of General Virology
dc.titleCharacterization of Barmah Forest virus pathogenesis in a mouse model
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume95
dc.date.issued2014
local.identifier.absfor110316 - Pathology
local.identifier.absfor110309 - Infectious Diseases
local.identifier.ariespublicationU3488905xPUB4625
local.type.statusPublished Version
local.contributor.affiliationHerrero, Lara, Griffith University
local.contributor.affiliationLidbury, Brett, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationBettadapura, Jayaram, Institute for Glycomics, Griffith University
local.contributor.affiliationPeng, Jian, Institute for Glycomics, Griffith University
local.contributor.affiliationHerrig, Belinda L, Sydney Medical School, University of Sydney
local.contributor.affiliationHey-Cunningham, William J., Sydney Medical School, University of Sydney
local.contributor.affiliationSheng, Kuo-Ching, Griffith University
local.contributor.affiliationZakhary, Andrew, University of Canberra
local.contributor.affiliationMahalingam, Suresh, Griffith University
local.description.embargo2037-12-31
local.bibliographicCitation.startpage2146
local.bibliographicCitation.lastpage2154
local.identifier.doi10.1099/vir.0.064733-0
local.identifier.absseo920109 - Infectious Diseases
dc.date.updated2015-12-11T09:05:21Z
local.identifier.scopusID2-s2.0-84907202361
local.identifier.thomsonID000344911900006
CollectionsANU Research Publications

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