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Sequence capture using PCR-generated probes: A cost-effective method of targeted high-throughput sequencing for nonmodel organisms

Penalba, Joshua V.; Smith, Lydia L.; Tonione, M.; Sass, Chodon; Hykin, Sarah M.; Skipwith, Phillip L.; McGuire, Jimmy A; Bowie, Rauri C K; Moritz, Craig


Recent advances in high-throughput sequencing library preparation and subgenomic enrichment methods have opened new avenues for population genetics and phylogenetics of nonmodel organisms. To multiplex large numbers of indexed samples while sequencing predominantly orthologous, targeted regions of the genome, we propose modifications to an existing, in-solution capture that utilizes PCR products as target probes to enrich library pools for the genomic subset of interest. The sequence capture...[Show more]

CollectionsANU Research Publications
Date published: 2014
Type: Journal article
Source: Molecular Ecology Resources
DOI: 10.1111/1755-0998.12249


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