Calcium dynamics, buffering, and buffer saturation in the boutons of dentate granule-cell axons in the Hilus
The axons of dentate gyrus granule cells form synapses in the hilus. Ca2+ signaling was investigated in the boutons of these axons using confocal fluorescence imaging. Boutons were loaded with various concentrations of the Ca2+ indicator Oregon Green BAPTA-1 by patch-clamping the cell bodies and allowing the dye to diffuse into the axon. Resting free [Ca2+] started at 74 nM, rose to ∼1 μM immediately after an action potential, and then decayed to rest with a time constant of 43 msec (all...[Show more]
|Collections||ANU Research Publications|
|Source:||Journal of Neuroscience|
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