Isolation of chromosomal regions controlling intersex development in a marsupial
-
Altmetric Citations
Santucciu, C; Gruetzner, Frank; Carvalho-Silva, D; Graves, Jennifer
Description
A marsupial (Sminthopsis douglasi) with bilateral intersexuality had a hemiscrotum on the right side and a hemi-pouch with nipples on the left. A normal female karyotype (2n = 14, XX) was present in cells from the right (male) side, while cells from the left (female) side initially had a female karyotype plus two dot-like chromosomes (2n = 14, XX + 2B). It is proposed that the dots represented a region deleted from the X chromosome that contains the "pouch-mammary/scrotum" (PMS) switch gene...[Show more]
dc.contributor.author | Santucciu, C | |
---|---|---|
dc.contributor.author | Gruetzner, Frank | |
dc.contributor.author | Carvalho-Silva, D | |
dc.contributor.author | Graves, Jennifer | |
dc.date.accessioned | 2015-12-13T22:29:27Z | |
dc.date.available | 2015-12-13T22:29:27Z | |
dc.identifier.issn | 1424-8581 | |
dc.identifier.uri | http://hdl.handle.net/1885/74727 | |
dc.description.abstract | A marsupial (Sminthopsis douglasi) with bilateral intersexuality had a hemiscrotum on the right side and a hemi-pouch with nipples on the left. A normal female karyotype (2n = 14, XX) was present in cells from the right (male) side, while cells from the left (female) side initially had a female karyotype plus two dot-like chromosomes (2n = 14, XX + 2B). It is proposed that the dots represented a region deleted from the X chromosome that contains the "pouch-mammary/scrotum" (PMS) switch gene whose dosage determines development of a pouch and teats (two doses) or a scrotum (one dose). Mis-segregation early in embryonic development produced a lineage with one normal X and one deleted X (male side), and a lineage with a normal and deleted X, plus two copies of the deleted region (female side). The origin of the supernumerary elements was therefore investigated in the expectation that they may contain the long-sought pouch-mammary/scrotum switch gene. Several elements were microdissected, and amplified DNA was used for in situ hybridization, producing signals in five different chromosome regions including the X. This could represent a region of the X that contains, as well as PMS, repetitive DNA that is present also at other chromosomal sites. | |
dc.publisher | S Karger AG | |
dc.source | Cytogenetic and Genome Research | |
dc.subject | Keywords: animal cell; cell lineage; chromosome deletion; conference paper; controlled study; embryo development; gene amplification; gene dosage; gene switching; hermaphroditism; in situ hybridization; karyotype 46,XX; mammary gland; marsupial; nipple; nonhuman; p | |
dc.title | Isolation of chromosomal regions controlling intersex development in a marsupial | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.description.refereed | Yes | |
local.identifier.citationvolume | 101 | |
dc.date.issued | 2003 | |
local.identifier.absfor | 060403 - Developmental Genetics (incl. Sex Determination) | |
local.identifier.ariespublication | MigratedxPub4272 | |
local.type.status | Published Version | |
local.contributor.affiliation | Santucciu, C, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Gruetzner, Frank, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Carvalho-Silva, D, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Graves, Jennifer, College of Medicine, Biology and Environment, ANU | |
local.bibliographicCitation.startpage | 224 | |
local.bibliographicCitation.lastpage | 228 | |
local.identifier.doi | 10.1159/000074341 | |
dc.date.updated | 2015-12-11T08:49:52Z | |
local.identifier.scopusID | 2-s2.0-0346058032 | |
Collections | ANU Research Publications |
Download
Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.
Updated: 17 November 2022/ Responsible Officer: University Librarian/ Page Contact: Library Systems & Web Coordinator